LOCUS       sequence-141321-         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid constitutively expresses gRNA-r11 from an AHD1 promoter
            using the insulated RGR design. Integrates in the W303 HIS locus and
            restores HIS function..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Gander MW, Vrana JD, Voje Jr WE, Carothers JM, Klavins E
  TITLE     Robust digital logic circuits in eukaryotic cells with CRISPR/dCas9 
            NOR gates
  JOURNAL   bioRxiv 041871
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            041871"
COMMENT     Sequence Label: pMOD8A-iRGR-r11
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(1..68)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     misc_RNA        complement(73..148)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(266..282)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(266..282)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(299..700)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
ORIGIN
        1 gtcccattcg ccatgccgaa gcatgttgcc cagccggcgc cagcgaggag gctgggacca
       61 tgccggccaa aagcaccgac tcggtgccac tttttcaagt tgataacgga ctagccttat
      121 tttaacttgc tatttctagc tctaaaaccg acaagcgtct ctttgcccgc agggggacgg
      181 gcgactatcc gacgtttccg tttcgacttg gccgaagcct tcgtctcttc agggatagat
      241 gatggcccgt ccctcaacct ttgtagatcc actagttcta gaatcctgta tatgagatag
      301 ttgattgtat gcttggtata gcttgaaata ttgtgcagaa aaagaaacaa ggaagaaagg
      361 gaacgagaac aatgacgagg aaacaaaaga ttaataattg caggtctatt tatacttgat
      421 agcaagacag caaacttttt tttatttcaa attcaagtaa ctggaaggaa ggccgtatac
      481 cgttgctcat tagagagtag tgtgcgtgaa tgaaggaagg aaaaagtttc gtgtgcttcg
      541 agatacccct catcagctct ggaacaacga catctgttgg tgctgtcttt gtcgttaatt
      601 ttttccttta gtgtcttcca tcattttttt gtcattgcgg atatggtgag acaacaacgg
      661 gggagagaga aaagaaaaaa aaagaaaaga agttgcatgc gcctattatt acttcaatag
      721 atggcaaatg gaaaaagggt agtgaaactt cgatatgatg atggctatca agtctagggc
      781 tacagtatta gttcgttatg taccaccatc aatgaggcag tgtaattggt gtagtcttgt
      841 ttagcccatt atgtcttgtc tggtatctgt tctattgtat atctcccctc cgccacctac
      901 atgttaggga gaccaacgaa ggtattatag gaatcccgat gtatgggttt ggttgccaga
      961 aaagaggaag tccatattgt acacc
//
