LOCUS       sequence-141322-         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid constitutively expresses gRNA-r11 from an AHD1 promoter
            using the insulated RGR design. Integrates in the W303 HIS locus and
            restores HIS function..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Gander MW, Vrana JD, Voje Jr WE, Carothers JM, Klavins E
  TITLE     Robust digital logic circuits in eukaryotic cells with CRISPR/dCas9 
            NOR gates
  JOURNAL   bioRxiv 041871
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            041871"
COMMENT     Sequence Label: pMOD8A-iRGR-r11
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(190..377)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(530..549)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     649..671
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(709..727)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        795..899
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtccagaaac cctatacctg tgtggacgtt aatcacttgc gattgtgtgg cctgttctgc
       61 tactgcttct gcctcttttt ctgggaagat cgagtgctct atcgctaggg gaccaccctt
      121 taaagagatc gcaatctgaa tcttggtttc atttgtaata cgctttacta gggctttctg
      181 ctctgtcatc tttgccttcg tttatcttgc ctgctcattt tttagtatat tcttcgaaga
      241 aatcacatta ctttatataa tgtataattc attatgtgat aatgccaatc gctaagaaaa
      301 aaaaagagtc atccgctagg tggaaaaaaa aaaatgaaaa tcattaccga ggcataaaaa
      361 aatatagagt gtactagagg aggccaagag taatagaaaa agaaaattgc gggaaaggac
      421 tgtgttatga cttccctgac taatgccgtg ttcaaacgat acctggcagt gactcctagc
      481 gctcaccaag ctcttaaaac ggtttaaacg aattatggtg cactctcagt acaatctgct
      541 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
      601 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
      661 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
      721 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
      781 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      841 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      901 tgagtattca acatttccgt gt
//
