LOCUS       sequence-141325-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid constitutively expresses gRNA-r10 from an AHD1 promoter
            using the RGR design. Integrates in the W303 HIS locus and restores 
            HIS function..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Gander MW, Vrana JD, Voje Jr WE, Carothers JM, Klavins E
  TITLE     Robust digital logic circuits in eukaryotic cells with CRISPR/dCas9 
            NOR gates
  JOURNAL   bioRxiv 041871
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            041871"
COMMENT     Sequence Label: pMOD8A-RGR-r10
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(6..73)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     misc_RNA        complement(78..153)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           complement(174..210)
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     primer_bind     complement(223..239)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(223..239)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(256..657)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
ORIGIN
        1 tatcagtccc attcgccatg ccgaagcatg ttgcccagcc ggcgccagcg aggaggctgg
       61 gaccatgccg gccaaaagca ccgactcggt gccacttttt caagttgata acggactagc
      121 cttattttaa cttgctattt ctagctctaa aacgagtcga atgtttatgt cacgacgagc
      181 ttactcgttt cgtcctcacg gactcatcag gtgacatttg tagatccact agttctagaa
      241 tcctgtatat gagatagttg attgtatgct tggtatagct tgaaatattg tgcagaaaaa
      301 gaaacaagga agaaagggaa cgagaacaat gacgaggaaa caaaagatta ataattgcag
      361 gtctatttat acttgatagc aagacagcaa actttttttt atttcaaatt caagtaactg
      421 gaaggaaggc cgtataccgt tgctcattag agagtagtgt gcgtgaatga aggaaggaaa
      481 aagtttcgtg tgcttcgaga tacccctcat cagctctgga acaacgacat ctgttggtgc
      541 tgtctttgtc gttaattttt tcctttagtg tcttccatca tttttttgtc attgcggata
      601 tggtgagaca acaacggggg agagagaaaa gaaaaaaaaa gaaaagaagt tgcatgcgcc
      661 tattattact tcaatagatg gcaaatggaa aaagggtagt gaaacttcga tatgatgatg
      721 gctatcaagt ctagggctac agtattagtt cgttatgtac caccatcaat gaggcagtgt
      781 aattggtgta gtcttgttta gcccattatg tcttgtctgg tatctgttct attgtatatc
      841 tcccctccgc cacctacatg ttagggagac caacgaaggt attataggaa tcccgatgta
      901 tgggtttggt tgccagaaaa gaggaa
//
