LOCUS       sequence-141326-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of PSAT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Mullarky E, Lucki NC, Beheshti Zavareh R, Anglin JL, Gomes AP, 
            Nicolay BN, Wong JC, Christen S, Takahashi H, Singh PK, Blenis J, 
            Warren JD, Fendt SM, Asara JM, DeNicola GM, Lyssiotis CA, Lairson 
            LL, Cantley LC
  TITLE     Identification of a small molecule inhibitor of 3-phosphoglycerate 
            dehydrogenase to target serine biosynthesis in cancers.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 16;113(7):1778-83. doi: 
            10.1073/pnas.1521548113. Epub 2016 Feb 1.
  PUBMED    26831078
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1521548113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-02-16- 16"; volume: "113"; issue: "7"; pages: "1778-83"
COMMENT     Sequence Label: pET28a-PSAT
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(101..148)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     144..162
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(215..232)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
       61 gcgtcccatt cgccaatccg gatatagttc ctcctttcag caaaaaaccc ctcaagaccc
      121 gtttagaggc cccaaggggt tatgctagtt attgctcagc ggtggcagca gccaactcag
      181 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
      241 cggccgcaag cttgtcgacg gagctcgaat tcggatccac tagtaacggc cgccagtgtg
      301 ctggaattcg cccttttgga tcctcatagc tgatgcatct ccaaaaattt tttcatgaag
      361 gcggccagct tctgaacgtc ttcaattgtg acagcattat acagagaggc ccggatgcct
      421 cccacagacc tatgcccttt caaggacaac atattgagtt caagagcttt atcaagaaat
      481 cttttttcta aagcatcatc tcctttggca ttgccaatgc ggaatggaat attcatcttg
      541 cttctatttt ggggctccac tggacaaacg tagaatcctt gagaattatc aataatctca
      601 taaattgttt gagatttgat ggagctaagc ttctccatgg ccgcggcacc tccattgttt
      661 ttaatccact ccagaaccaa gcccatgacg tagatgctga ngcatggagg cgtgttgtac
      721 aaggagctgt ttccagcctg caccttgtat tccaggaccg aggggcactc tcggagggca
      781 aaccccagca ggtcatcacg gacaatcacc acggtgaccc cagcagagcc aacattcttc
      841 tgggcaccag caaaaatcac accaaacttg gaaacatcca ctggcttgga caggaagttt
      901 gaggacatgt cacaaa
//
