LOCUS       sequence-141327-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid constitutively expresses gRNA-r10 from an AHD1 promoter
            using the RGR design. Integrates in the W303 HIS locus and restores 
            HIS function..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Gander MW, Vrana JD, Voje Jr WE, Carothers JM, Klavins E
  TITLE     Robust digital logic circuits in eukaryotic cells with CRISPR/dCas9 
            NOR gates
  JOURNAL   bioRxiv 041871
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            041871"
COMMENT     Sequence Label: pMOD8A-RGR-r10
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(194..381)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(534..553)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     653..675
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(713..731)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tatggtccag aaaccctata cctgtgtgga cgttaatcac ttgcgattgt gtggcctgtt
       61 ctgctactgc ttctgcctct ttttctggga agatcgagtg ctctatcgct aggggaccac
      121 cctttaaaga gatcgcaatc tgaatcttgg tttcatttgt aatacgcttt actagggctt
      181 tctgctctgt catctttgcc ttcgtttatc ttgcctgctc attttttagt atattcttcg
      241 aagaaatcac attactttat ataatgtata attcattatg tgataatgcc aatcgctaag
      301 aaaaaaaaag agtcatccgc taggtggaaa aaaaaaaatg aaaatcatta ccgaggcata
      361 aaaaaatata gagtgtacta gaggaggcca agagtaatag aaaaagaaaa ttgcgggaaa
      421 ggactgtgtt atgacttccc tgactaatgc cgtgttcaaa cgatacctgg cagtgactcc
      481 tagcgctcac caagctctta aaacggttta aacgaattat ggtgcactct cagtacaatc
      541 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
      601 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
      661 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
      721 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      781 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      841 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
//
