LOCUS       sequence-141330-        7851 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a truncation of Hook3 in bacteria for purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7851)
  AUTHORS   Schroeder CM, Vale RD
  TITLE     Assembly and activation of dynein-dynactin by the cargo adaptor 
            protein Hook3.
  JOURNAL   J Cell Biol. 2016 Aug 1;214(3):309-18. doi: 10.1083/jcb.201604002.
  PUBMED    27482052
REFERENCE   2  (bases 1 to 7851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2016-08-1- 1"; volume: "214"; 
            issue: "3"; pages: "309-18"
COMMENT     Sequence Label: pET28a-Hook3 aa 1-552
FEATURES             Location/Qualifiers
     source          1..7851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(587..1669)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1631..1650
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1670..1747)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1953..1972
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2056..2075
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2056..2074
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2075..2099
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2114..2136
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2154..2171
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2181..2198
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             2202..2234
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(2250..2273,2274..2309,2310..2333)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             join(2340..2342,2343..3053)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2340..3053
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2370..2398)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2993..3013
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             3060..3080
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4767..4784
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4837..4855)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4851..4898
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4935..5390
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5022..5041)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5232..5253
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5483..6298)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5483..6298)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6206..6225
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6290..6309
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6420..7008
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6909..6928
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7162..7179
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7194..7336
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7262..7281)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7422..7444
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7438..7629)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt
       61 ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga
      121 cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc
      181 agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac
      241 ccgtggggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg
      301 accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc
      361 gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg
      421 cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat
      481 gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga
      541 tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt
      601 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
      661 cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct
      721 gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc
      781 ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt
      841 cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa
      901 tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga
      961 tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt
     1021 cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac
     1081 gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca
     1141 atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt
     1201 tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt
     1261 ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt
     1321 gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg
     1381 acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg
     1441 acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg
     1501 ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt
     1561 tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat
     1621 aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc
     1681 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga
     1741 tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
     1801 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
     1861 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
     1921 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
     1981 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc
     2041 tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc
     2101 ctctagaaat aattttgttt aactttaaga aggagatata catgggcagc agccatcatc
     2161 atcatcatca cagcagcggc ctggtgccgc gcggcagcca tatggctagc atgactggtg
     2221 gacagcaaat gggtcgcgga tccgaattct ggagtcatcc acaatttgag aagggcggcg
     2281 gcagtggcgg cggcagtggc ggcagtgcct ggagccaccc gcagttcgaa aaaggcagta
     2341 tgagcaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
     2401 atgttaatgg gcacaaattt tctgtccgtg gagagggtga aggtgatgct acaaacggaa
     2461 aactcaccct taaatttatt tgcactactg gaaaactacc tgttccgtgg ccaacacttg
     2521 tcactactct gacctatggt gttcaatgct tttcccgtta tccggatcac atgaaacggc
     2581 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaacgcact atatctttca
     2641 aagatgacgg gacctacaag acgcgtgctg aagtcaagtt tgaaggtgat acccttgtta
     2701 atcgtatcga gttaaagggt attgatttta aagaagatgg aaacattctt ggacacaaac
     2761 tcgaatacaa ctttaactca cacaatgtat acatcacggc agacaaacaa aagaatggaa
     2821 tcaaagctaa cttcaaaatt cgccacaacg ttgaagatgg ttccgttcaa ctagcagacc
     2881 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
     2941 tgtcgacaca atctgtcctt tcgaaagatc ccaacgaaaa gcgtgaccac atggtccttc
     3001 ttgagtttgt aactgctgct gggattacac atggcatgga tgagctctac aaaggatccg
     3061 aaaatctgta ttttcagggg ggtggaggtg gcatgttcag cgtagagtcg ctggagcggg
     3121 cggagctgtg cgagagcctc ctcacttgga tccagacatt taatgtggat gcaccatgcc
     3181 agaccgtgga agatttaacg aatggggttg tgatggccca ggttcttcaa aagatagatc
     3241 ctgcatattt tgatgaaaat tggctaaaca gaatcaaaac tgaagtagga gataattgga
     3301 ggctaaagat aagcaattta aagaaaattt taaaaggaat cttggattat aatcatgaga
     3361 ttttaggaca gcaaattaat gactttaccc ttcctgatgt gaaccttatt ggggagcatt
     3421 ctgatgcagc agagcttgga aggatgcttc agctcatctt aggctgtgct gtgaactgtg
     3481 aacagaagca agagtacatc caagccatta tgatgatgga ggaatctgtt caacatgttg
     3541 tcatgacagc cattcaagag ctgatgagta aagaatctcc tgtctctgct ggaaatgatg
     3601 cctatgttga ccttgatcgt cagctgaaga aaactacaga ggaactaaat gaagctttgt
     3661 cagcaaagga agaaattgct caaagatgcc atgaactgga tatgcaggtt gcagcattgc
     3721 aggaagagaa aagtagtttg ttggcagaga atcaggtatt aatggaaaga ctcaatcaat
     3781 ctgattctat agaagaccct aacagtccag caggaagaag gcatttgcag ctccagactc
     3841 aattagaaca gctccaagaa gaaacattca gactagaagc agccaaagat gattatcgaa
     3901 tacgttgtga agagttagaa aaggagatct ctgaacttcg gcaacagaat gatgaactga
     3961 ccactttggc agatgaagct cagtctctga aagatgagat cgacgtgctg agacattctt
     4021 ctgataaagt atctaaacta gaaggtcaag tagaatctta taaaaagaag ctagaagacc
     4081 ttggtgattt aaggcggcag gttaaactct tagaagagaa gaataccatg tatatgcaga
     4141 atactgtcag tctagaggaa gagttaagaa aggccaacgc agcgcgaagt caacttgaaa
     4201 cctacaagag acaggtagta gaactacaaa acagattatc cgaagaatca aagaaagcag
     4261 ataaactaga ttttgaatat aagcggctaa aagaaaaagt tgacagtctt caaaaagaaa
     4321 aggacaggct gagaacagaa agggattctc tgaaggaaac cattgaagag cttcgttgtg
     4381 tacaagctca agaagggcag ctcacaacac aagggttaat gcctcttgga agtcaggagt
     4441 cttcagacag tctagctgca gagattgtta cacctgaaat cagggagaaa cttattcgtc
     4501 ttcagcatga gaataagatg ttaaagctta accaagaagg ttcggacaat gaaaaaatag
     4561 ccttattgca gagccttcta gatgatgcaa atctacgcaa gaatgaactg gagacagaga
     4621 ataggctggt gaatcaaaga cttctggaag tacagtcaca agttgaagaa ttacaaaaat
     4681 ctttacagga tcaaggctca aaagcagaag attcagtcct tctaaaaaag aagcttgaag
     4741 aacatctata agcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta
     4801 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
     4861 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
     4921 gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     4981 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     5041 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     5101 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     5161 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     5221 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     5281 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     5341 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt caggtggcac
     5401 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     5461 gtatccgctc atgaattaat tcttagaaaa actcatcgag catcaaatga aactgcaatt
     5521 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag
     5581 aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga
     5641 ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg
     5701 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt
     5761 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca
     5821 aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag
     5881 gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa
     5941 tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg
     6001 cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag
     6061 gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc
     6121 tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga
     6181 ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat
     6241 ccatgttgga atttaatcgc ggcctagagc aagacgtttc ccgttgaata tggctcataa
     6301 caccccttgt attactgttt atgtaagcag acagttttat tgttcatgac caaaatccct
     6361 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6421 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6481 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6541 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     6601 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6661 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6721 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6781 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6841 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6901 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6961 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7021 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     7081 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     7141 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     7201 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca
     7261 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
     7321 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     7381 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     7441 gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg
     7501 gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc
     7561 cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg
     7621 tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat
     7681 gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga
     7741 acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca
     7801 gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta g
//
