LOCUS       sequence-141339-       10043 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes for the QF-GR recombinant gene, splice linker, and mCherry 
            reporter gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10043)
  AUTHORS   Monsalve GC, Yamamoto KR, Ward JD
  TITLE     A New Tool for Inducible Gene Expression in Caenorhabditis elegans.
  JOURNAL   Genetics. 2019 Feb;211(2):419-430. doi: 10.1534/genetics.118.301705.
            Epub 2018 Nov 30.
  PUBMED    30504365
REFERENCE   2  (bases 1 to 10043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.118.301705"; journalName: "Genetics"; date: 
            "2019-02"; volume: "211"; issue: "2"; pages: "419-430"
COMMENT     Sequence Label: pGM32DEST
FEATURES             Location/Qualifiers
     source          1..10043
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2187..2209
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2206..2222
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    2276..2400
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2425..2430,2431..2448,2449..2455)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2509..3168
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2575..2594)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3510..3815
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3694..3713
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3856..3980)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(8023..8056)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(9649..9666)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9649..9665)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9658..9680)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt gcatgctcca gtgtggtgga attctgcaga tatcaacaag
     2281 tttgtacaaa aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta
     2341 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg
     2401 gcggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg
     2461 attttgagtt aggatccggc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
     2521 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
     2581 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
     2641 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
     2701 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata
     2761 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
     2821 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
     2881 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
     2941 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
     3001 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
     3061 atgccgctgg cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg
     3121 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaaag atctggatcc
     3181 ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga
     3241 atatatactg atatgtatac ccgaagtatg tcaaaaagag gtgtgctatg aagcagcgta
     3301 ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat
     3361 ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg
     3421 gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc
     3481 ttttgctgac gagaacaggg actggtgaaa tgcagtttaa ggtttacacc tataaaagag
     3541 agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac
     3601 ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt
     3661 acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg
     3721 tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca
     3781 aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccgtt atacacagcc
     3841 agtctgcagg tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
     3901 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
     3961 gctttcttgt acaaagtggt tgatatccag cacagtggcg gccgctcgag gcgcgcctct
     4021 agaggatccc cgggattggc caaaggaccc aaaggtatgt ttcgaatgat actaacataa
     4081 catagaacat tttcaggagg acccttggct agccatgcca ccaaaacgca agacactcaa
     4141 tgccgctgcc gaagccaatg cccacgctga tggccatgct gatggcaatg ctgatggtca
     4201 tgtcgctaac actgcagcaa gcagcaacaa cgcccgtttt gcggacttga ccaacattga
     4261 cacacccggc ctcggcccta ccacgacgac gttacttgtc gagcccgctc gttcgaaacg
     4321 ccagagagtc tcgagggcct gtgatcagtg tcgagctgca cgtgaaaagt gtgatggaat
     4381 ccagccggct tgcttcccct gtgtgtcgca gggccggtcg tgtacctacc aggccagtcc
     4441 caagaagcga ggagtccaga cgggctacat ccgcactctc gaactggctc tggcttggat
     4501 gttcgagaac gttgcccgca gcgaggacgc cctccacaat cttttggtcc gtgatgctgg
     4561 ccagggcagc gctctcctgg tcggcaaaga ctcgcctgct gcagaacgcc tccatgcaag
     4621 atgggcgacg agtcgagtca acaaaagcat cacccgtctt ctctcaggtc aggccgcaca
     4681 agatccatct gaagacggcc aatccccgtc cgaagacata aatgtccaag atgccggggc
     4741 aaagacatcc gacttccctc atgcgcctca cttgactttc tcggcgccta agtctagtac
     4801 agctgagaca cgcactctac caggcccggt ccgaccacct atttcggcaa acaccctgga
     4861 aaacaacctt cagccagatg gaaccgggat agggaagcta ccacccaatc attggcgcct
     4921 gctggatatc tacttttcct acacgcattc ttggctccct atcctcgaga agaaagacat
     4981 gtaccaagca ttgtaccagt actctgaaca aggctcgttg cttccctctg cgaatgtcga
     5041 gtctggcgtt catgccgagc tttggagcgc gctcgccctg gcgtccttcc aggctgctgc
     5101 tactgctgca tcgagtgcta cgggtccagc ttcagctgct catggccatg acaatgccat
     5161 caatccttca cctgcagaca tatctgacac agcccgaaag ctcatacctt tggaaagcgg
     5221 gccgttccag gttcagcact gcagagcgtt gctgcttctt tgtctcgtaa gccttgggcg
     5281 ggatgattgg gagtctgctt ggttgctggt tggctttgcg gtccgcgtcc tacttgttgt
     5341 tcgcacccag ttgcctcctg atgatgaccg gccacgacca agaatgcgtg cgctgctcgt
     5401 cgcgtgcttc atcgtggata ccattgtgtc tatgagacac aacgtgccgg cccatctcaa
     5461 gccagacgac attgcggatc tgccgttacc tgaagacggt caagatcaat gggagccgtg
     5521 gacaccatgt gagggcttag gcggtgaaca caccatgctg caaatgttga ggaacccggc
     5581 atacccttta agcacattca accacctata tggcgtgacc aagctggttg ctttggagct
     5641 tctgccaaga atacgaacat cttcacagaa cgctcccttg gagttcaggt cgcggttgca
     5701 gcaggtaatc ggccacaatt ctcccttcag cgtctttgtc ctttcccagg atacagcatc
     5761 ggcttttgtg cctactgcat accttacccg taccgtttat ttatgggcag ctgccttttc
     5821 tgagcctctc aacgaacact actcgcatct tctgatcgaa actcttgatc agtatcagaa
     5881 gcggtttggt acatatgcaa tcccacctct gatcccttct cttctagact cccttcttgc
     5941 tttaaagaaa caatcacatt cttcagagcg gcatcgaagg cacttggaag agcttttccc
     6001 cgcctactcc tccatttggc ctcggggagg ccgacacagc aatactggcc tccaacccat
     6061 acgacaactt gagcttccgc cgactgcgac tgccactgca agtatcatgc cccatgtcat
     6121 ggaacagccc ctgtcaacgt caataaatcc ggtcaatgat cggtttaatg gaataccgaa
     6181 tcctaccccc tacaatagcg atgcggccct agacgcaatt actcaaacca atgactacgg
     6241 atcagtcaat acccatggca ttctcagtac atatccacca cctgccaccc acttgaacga
     6301 ggcctcggtg gcccttgcgc ctggaggtgc tcctcccaga ccacccccgc catacgttga
     6361 tagtacaacg aaccatcctc cttaccatag taacctcgtc cccatggcca atttcggata
     6421 ttcgactgta gactatgatg cgatggtgga tgatctggca tcgatcgagt acacggatgc
     6481 ggttgacgtt gatccgcagt tcatgacgaa cctcgggttt gtcccagggt gtaattttag
     6541 cgatatcaac acgtatgagc aagttcctgc aacgttacca caactcaccc ctaccctggt
     6601 gtcactgttg gaggttattg aacctgaagt gttatatgca ggatatgata gctctgttcc
     6661 agactcaact tggaggatca tgactacgct caacatgtta ggagggcggc aagtgattgc
     6721 agcagtgaaa tgggcaaagg caataccagg tttcaggaac ttacacctgg atgaccaaat
     6781 gaccctactg cagtactcct ggatgtttct tatggcattt gctctggggt ggagatcata
     6841 tagacaatca agtgcaaacc tgctgtgttt tgctcctgat ctgattatta atgagcagag
     6901 aatgactcta ccctgcatgt acgaccaatg taaacacatg ctgtatgttt cctctgagtt
     6961 acacaggctt caggtatctt atgaagagta tctctgtatg aaaaccttac tgcttctctc
     7021 ttcagttcct aaggacggtc tgaagagcca agagctattt gatgaaatta gaatgaccta
     7081 catcaaagag ctaggaaaag ccattgtcaa gagggaagga aactccagcc agaactggca
     7141 gcggttttat caactgacaa aactcttgga ttctatgcat gaagtggttg aaaatctcct
     7201 taactattgc ttccaaacat ttttggataa gaccatgagt attgaattcc ccgagatgtt
     7261 agctgaaatc atcaccaatc agataccaaa atattcaaat ggaaatatca aaaaacttct
     7321 gtttcatcaa aagtaggcta gcgtcgacgg taccgctgtc tcatcctact ttcacctagt
     7381 taactgcttg tcttaaaatc tatgcttctc tttagtatct aaaattttcc tagaagctta
     7441 caagtatata aatggtctct tctcaataaa ggttgtatat ttattcatct tattgaatct
     7501 gccatttcct cgtttttgcg agtttatata ccttccaatt ttctttctat tgtattttca
     7561 acttctaatt ttaattcagg gaaactgctt caacgcatca tggtctcaaa gggtgaagaa
     7621 gataacatgg caattattaa agagtttatg cgtttcaagg tgcatatgga gggatctgtc
     7681 aatgggcatg agtttgaaat tgaaggtgaa ggagaaggcc gaccatatga gggaacacaa
     7741 accgcaaaac taaaggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat
     7801 tttcaggtaa ctaaaggcgg accattacca ttcgcctggg acatcctctc tccacagttc
     7861 atgtatggaa gtaaagctta tgttaaacat ccggcagata taccagatta tttgaaactt
     7921 tcattcccgg agggttttaa gtgggaacgc gtaatgaatt ttgaagacgg aggagttgtt
     7981 acagtgacgc aagactcaag gtaagtttaa acagttcggt acgaagttcc tatactttct
     8041 agagaatagg aacttctaac taaccataca tatttaaatt ttcagcctcc aagatggaga
     8101 atttatttat aaagtcaaac ttcgaggaac gaatttcccc tcggatggac ctgttatgca
     8161 gaagaagact atgggatggg aagcttcaag tgaaagaatg taccctgaag acggtgctct
     8221 taagggagag attaaacaac gtcttaaatt gaaagatgga ggacattacg atgctgaggt
     8281 aagtttaaac atgattttac taactaacta atctgattta aattttcagg tgaagacaac
     8341 ttacaaagcc aaaaaaccag ttcagctgcc aggagcgtac aatgttaata ttaaactgga
     8401 tatcacctcc cacaacgagg attacactat cgttgagcaa tatgaaagag ctgaagggcg
     8461 gcactcgaca ggtggcatgg atgaattgta taagtaagag ctccgcatcg gccgctgtca
     8521 tcagatcgcc atctcgcgcc cgtgcctctg acttctaagt ccaattactc ttcaacatcc
     8581 ctacatgctc tttctccctg tgctcccacc ccctattttt gttattatca aaaaaacttc
     8641 ttcttaattt ctttgttttt tagcttcttt taagtcacct ctaacaatga aattgtgtag
     8701 attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca
     8761 ttaataataa ttctatccca aaatctacac aatgttctgt gtacacttct tatgtttttt
     8821 ttacttctga taaatttttt ttgaaacatc atagaaaaaa ccgcacacaa aataccttat
     8881 catatgttac gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc
     8941 atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt atttttggaa tttttcaatc
     9001 aagtgaaagt ttatgaaatt aattttcctg cttttgcttt ttgggggttt cccctattgt
     9061 ttgtcaagag tttcgaggac ggcgtttttc ttgctaaaat cacaagtatt gatgagcacg
     9121 atgcaagaaa gatcggaaga aggtttgggt ttgaggctca gtggaaggtg agtagaagtt
     9181 gataatttga aagtggagta gtgtctatgg ggtttttgcc ttaaatgaca gaatacattc
     9241 ccaatatacc aaacataact gtttcctact agtcggccgt acgggccctt tcgtctcgcg
     9301 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     9361 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     9421 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     9481 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcggccttaa
     9541 ttaattctag ctagtgggca gatcttcgaa tgcatcgcgc gcaccgtacg tctcgaggaa
     9601 ttcctgcagg tcgactctag aggatccccg ggtaccgagc tcgaattcac tggccgtcgt
     9661 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     9721 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     9781 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
     9841 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     9901 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     9961 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
    10021 accgtcatca ccgaaacgcg cga
//
