LOCUS       sequence-141344-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  KillerOrange gene for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Sarkisyan KS, Zlobovskaya OA, Gorbachev DA, Bozhanova NG, Sharonov 
            GV, Staroverov DB, Egorov ES, Ryabova AV, Solntsev KM, Mishin AS, 
            Lukyanov KA
  TITLE     KillerOrange, a Genetically Encoded Photosensitizer Activated by 
            Blue and Green Light.
  JOURNAL   PLoS One. 2015 Dec 17;10(12):e0145287. doi: 
            10.1371/journal.pone.0145287. eCollection 2015.
  PUBMED    26679300
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-12-17"; volume: "10(12):e0145287. doi"; pages: " 
            10.1371/journal.pone.0145287. eCollection 2015"
COMMENT     Sequence Label: KillerOrange /pQE-30
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    15..31
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             50..61
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             80..97
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             104..814
                     /codon_start=1
                     /product="orange fluorescent protein KillerOrange, a 
                     genetically-encoded photosensitizer derived from KillerRed 
                     (Sarkisyan et al., 2015)"
                     /label=KillerOrange
                     /note="mammalian codon-optimized"
                     /translation="ECGPALFQSDMTFKIFIDGEVNGQKFTIVADGSSKFPHGDFNVHA
                     VCETGKLPMSWKPICHLIQWGEPFFARYPDGISHFAQECFPEGLSIDRTVRFENDGTMT
                     SHHTYELSDTCVVSRITVNCDGFQPDGPIMRDQLVDILPSETHMFPHGPNAVRQLAFIG
                     FTTADGGLMMGHLDSKMTFNGSRAIEIPGPHFVTIITKQMRDTSDKRDHVCQREVAHAH
                     SVPRITSAIGSDQD"
     terminator      836..930
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 tataatagat tcaattgtga gcggataaca atttcacaca gaattcatta aagaggagaa
       61 attaactatg agaggatcgc atcaccatca ccatcacgga tccgagtgcg gccccgccct
      121 gttccagagc gacatgacct tcaaaatctt catcgacggc gaggtgaacg gccagaagtt
      181 caccatcgtg gccgacggca gcagcaagtt cccccacggc gacttcaacg tgcacgccgt
      241 gtgcgagacc ggcaagctgc ccatgagctg gaagcccatc tgccacctga tccagtgggg
      301 cgagcccttc ttcgcccgct accccgacgg catcagccat ttcgcccagg agtgcttccc
      361 cgagggcctg agcatcgacc gcaccgtgcg cttcgagaac gacggcacca tgaccagcca
      421 ccacacctac gagctgagcg acacctgcgt ggtgagccgc atcaccgtga actgcgacgg
      481 cttccagccc gacggcccca tcatgcgcga ccagctggtg gacatcctgc ccagcgagac
      541 ccacatgttc ccccacggcc ccaacgccgt gcgccagctg gccttcatcg gcttcaccac
      601 cgccgacggc ggcctgatga tgggccacct cgacagcaag atgaccttca acggcagccg
      661 cgccatcgag atccccggcc cacacttcgt gaccatcatc accaagcaga tgagggacac
      721 cagcgacaag cgcgaccacg tgtgccagcg cgaggtggcc cacgcccaca gcgtgccccg
      781 catcaccagc gccatcggta gcgaccagga ttgaaagctt aattagctga gcttggactc
      841 ctgttgatag atccagtaag aacctcagaa ctccatctgg atttgttcag aacgctcggt
      901 tgccgccggg cgttttttat tggtgagaat cc
//
