LOCUS       sequence-141351-        8466 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressed sfGFP-tagged FIP3 in E. coli for protein production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8466)
  AUTHORS   Schroeder CM, Ostrem JM, Hertz NT, Vale RD
  TITLE     A Ras-like domain in the light intermediate chain bridges the dynein
            motor to a cargo-binding region.
  JOURNAL   Elife. 2014 Oct 1;3:e03351. doi: 10.7554/eLife.03351.
  PUBMED    25272277
REFERENCE   2  (bases 1 to 8466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-10-1"; volume: "3:e03351. doi"; pages: " 
            10.7554/eLife.03351"
COMMENT     Sequence Label: pET28a-FIP3
FEATURES             Location/Qualifiers
     source          1..8466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(587..1669)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1631..1650
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1670..1747)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1953..1972
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2056..2075
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2056..2074
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2075..2099
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2114..2136
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2154..2171
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2181..2198
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             2202..2234
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(2250..2273,2274..2309,2310..2333)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             join(2340..2342,2343..3053)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2340..3053
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2370..2398)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2993..3013
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             3060..3080
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5382..5399
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5452..5470)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5466..5513
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5550..6005
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5637..5656)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5847..5868
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6098..6913)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6098..6913)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6821..6840
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6905..6924
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7035..7623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7524..7543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7777..7794
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7809..7951
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7877..7896)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8037..8059
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8053..8244)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt
       61 ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga
      121 cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc
      181 agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac
      241 ccgtggggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg
      301 accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc
      361 gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg
      421 cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat
      481 gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga
      541 tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt
      601 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
      661 cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct
      721 gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc
      781 ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt
      841 cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa
      901 tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga
      961 tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt
     1021 cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac
     1081 gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca
     1141 atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt
     1201 tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt
     1261 ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt
     1321 gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg
     1381 acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg
     1441 acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg
     1501 ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt
     1561 tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat
     1621 aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc
     1681 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga
     1741 tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
     1801 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
     1861 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
     1921 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
     1981 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc
     2041 tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc
     2101 ctctagaaat aattttgttt aactttaaga aggagatata catgggcagc agccatcatc
     2161 atcatcatca cagcagcggc ctggtgccgc gcggcagcca tatggctagc atgactggtg
     2221 gacagcaaat gggtcgcgga tccgaattct ggagtcatcc acaatttgag aagggcggcg
     2281 gcagtggcgg cggcagtggc ggcagtgcct ggagccaccc gcagttcgaa aaaggcagta
     2341 tgagcaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
     2401 atgttaatgg gcacaaattt tctgtccgtg gagagggtga aggtgatgct acaaacggaa
     2461 aactcaccct taaatttatt tgcactactg gaaaactacc tgttccgtgg ccaacacttg
     2521 tcactactct gacctatggt gttcaatgct tttcccgtta tccggatcac atgaaacggc
     2581 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaacgcact atatctttca
     2641 aagatgacgg gacctacaag acgcgtgctg aagtcaagtt tgaaggtgat acccttgtta
     2701 atcgtatcga gttaaagggt attgatttta aagaagatgg aaacattctt ggacacaaac
     2761 tcgaatacaa ctttaactca cacaatgtat acatcacggc agacaaacaa aagaatggaa
     2821 tcaaagctaa cttcaaaatt cgccacaacg ttgaagatgg ttccgttcaa ctagcagacc
     2881 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
     2941 tgtcgacaca atctgtcctt tcgaaagatc ccaacgaaaa gcgtgaccac atggtccttc
     3001 ttgagtttgt aactgctgct gggattacac atggcatgga tgagctctac aaaggatccg
     3061 aaaatctgta ttttcagggg ggtggaggtg gtaccatggc gtcggccccg ccggcctcgc
     3121 ccccgggctc ggagccgccg gggcccgacc cggagccggg cgggccggac gggccggggg
     3181 cggcgcaact ggctccgggc cctgcggagc tacgcctcgg agcgcccgtc ggcggccccg
     3241 acccgcagtc cccgggcctg gatgagcctg cgcccggggc cgctgcagat ggcggggcgc
     3301 gttggagcgc cgggccggcc ccggggctgg agggaggccc gcgagacccc gggccgtccg
     3361 ccccgccgcc gcgctccggc ccgcgggggc agcttgcgag ccccgacgcc ccgggcccag
     3421 ggccgcgctc cgaagcgccg cttccagaac tcgacccgtt gttctcctgg actgaggagc
     3481 ccgaggagtg tggccccgcg agctgcccgg agagcgcgcc tttccgcttg caggggtcca
     3541 gcagcagcca ccgagcgcgg ggcgaggtcg acgtcttctc tcccttcccc gcgcccacgg
     3601 cgggcgagct ggcgctggag caaggtcccg ggtccccgcc gcagccctcg gacctcagcc
     3661 agacccaccc ccttccgagc gagcccgtgg ggagtcagga ggacggcccc cgcctccgag
     3721 ccgtgttcga tgccctggac ggggatgggg acggtttcgt ccgcatcgag gacttcatcc
     3781 agtttgctac ggtctacggg gcagagcagg tgaaggactt aactaagtac ttggatccca
     3841 gtgggctcgg cgtgatcagc tttgaagact tctaccaagg gatcacagcc atcagaaacg
     3901 gagatcctga tggccagtgc tacggtggtg tcgcttctgc ccaagatgag gagcccctgg
     3961 cctgcccgga cgagttcgat gacttcgtca cctatgaggc caacgaggtg acggacagcg
     4021 cgtacatggg ctccgagagc acctacagtg agtgtgagac cttcacggac gaggacacca
     4081 gcaccctggt gcaccctgag ctgcaacctg aaggggacgc agacagtgcc ggcggctcgg
     4141 ccgtgccctc tgagtgcctg gacgccatgg aggagcccga ccatggtgcc ctgctgctgc
     4201 tcccaggcag gcctcacccc catggccagt ctgtcatcac ggtgatcggg ggcgaggagc
     4261 actttgagga ctacggtgaa ggcagtgagg cggagctgtc cccagagacc ctatgcaacg
     4321 ggcagctggg ctgcagtgac cccgctttcc tcacgcccag tccgacaaag cggctctcca
     4381 gcaagaaggt ggcaaggtac ctgcaccagt caggggccct gaccatggag gccctggagg
     4441 acccttcccc cgagctcatg gagggcccag aggaggacat tgctgacaag gttgtcttcc
     4501 tggaaaggcg tgtgctggag ctggaaaagg acacggcagc caccggtgag caacacagcc
     4561 gcctgaggca ggagaacctg cagctggtgc acagagcaaa cgccctggag gagcagctga
     4621 aggagcagga gctgagagcc tgcgagatgg tcctggaaga gacccggcgt cagaaggagc
     4681 tcctgtgcaa gatggagagg gagaagagca ttgagatcga gaacctgcag accaggctac
     4741 agcaactgga cgaggagaac agtgaactcc ggtcctgcac gccctgtctg aaggccaaca
     4801 ttgagcgtct ggaggaggag aagcagaagc tgttggatga gatagagtcg ctgacgctgc
     4861 ggctcagtga agagcaggag aacaagagga gaatggggga caggctgagt cacgagaggc
     4921 accagttcca gagggacaag gaggccaccc aggagctgat cgaggacctc cgaaagcagc
     4981 tggagcacct gcagctcctc aagctggagg ccgagcagcg gcggggccgc agcagcagca
     5041 tgggcctgca ggagtaccac agccgcgccc gggagagcga gctggagcag gaggtccgca
     5101 ggctgaagca ggacaaccgc aacctgaagg agcagaacga ggagctgaac gggcagatca
     5161 ttaccctcag catccagggc gccaagagcc tcttctccac agccttctct gagtccctgg
     5221 ctgcagagat cagctccgtc tcccgagatg agctcatgga ggcgattcag aagcaggagg
     5281 agatcaactt ccgcctgcag gactacatcg acaggatcat cgtggccatc atggagacca
     5341 acccgtccat cctggaggtc aagtaagcgg ccgcactcga gcaccaccac caccaccact
     5401 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
     5461 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
     5521 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     5581 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     5641 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     5701 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     5761 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     5821 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     5881 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     5941 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     6001 aatttcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     6061 aatacattca aatatgtatc cgctcatgaa ttaattctta gaaaaactca tcgagcatca
     6121 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt
     6181 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc
     6241 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa
     6301 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa
     6361 gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat
     6421 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc
     6481 gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg
     6541 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg
     6601 ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct
     6661 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa
     6721 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc
     6781 catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc
     6841 catataaatc agcatccatg ttggaattta atcgcggcct agagcaagac gtttcccgtt
     6901 gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc
     6961 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     7021 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     7081 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7141 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     7201 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7261 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7321 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7381 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     7441 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7501 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7561 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     7621 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     7681 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     7741 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     7801 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     7861 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     7921 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     7981 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     8041 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta
     8101 aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag
     8161 ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag
     8221 ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg
     8281 ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca
     8341 tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca
     8401 gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca
     8461 gggtag
//
