LOCUS       sequence-141360-         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid expressing mic-his tag phosphorylation-deficient hHSF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Budzynski MA, Puustinen MC, Joutsen J, Sistonen L
  TITLE     Uncoupling Stress-Inducible Phosphorylation of Heat Shock Factor 1 
            from Its Activation.
  JOURNAL   Mol Cell Biol. 2015 Jul;35(14):2530-40. doi: 10.1128/MCB.00816-14. 
            Epub 2015 May 11.
  PUBMED    25963659
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00816-14"; journalName: "Mol Cell Biol"; date: 
            "2015-07"; volume: "35"; issue: "14"; pages: "2530-40"
COMMENT     Sequence Label: pcDNA-HSF1-Delta∼PRD
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacgggc cctctagact
      121 cgagcggccg ccactgtgct ggatatctgc agaattcgag atggatctgc ccgtgggccc
      181 cggcgcggcg gggcccagca acgtcccggc cttcctgacc aagctgtgga ccctcgtgag
      241 cgacccggac accgacgcgc tcatctgctg gagcccgagc gggaacagct tccacgtgtt
      301 cgaccagggc cagtttgcca aggaggtgct gcccaagtac ttcaagcaca acaacatggc
      361 cagcttcgtg cggcagctca acatgtatgg cttccggaaa gtggtccaca tcgagcaggg
      421 cggcctggtc aagccagaga gagacgacac ggagttccag cacccatgct tcctgcgtgg
      481 ccaggagcag ctccttgaga acatcaagag gaaagtgacc agtgtgtcca ccctgaagag
      541 tgaagacata aagatccgcc aggacagcgt caccaagctg ctgacggacg tgcagctgat
      601 gaaggggaag caggagtgca tggactccaa gctcctggcc atgaagcatg agaatgaggc
      661 tctgtggcgg gaggtggcca gccttcggca gaagcatgcc cagcaacaga aagtcgtcaa
      721 caagctcatt cagttcctga tctcactggt gcagtcaaac cggatcctgg gggtgaagag
      781 aaagatcccc ctgatgctga acgacagtgg ctcagcacat tccatgccca agtatagccg
      841 gcagttcgcc ctggagcacg tccacggctc gggcccctac tcggccccct ccccagccta
      901 cagcagctcc agcc
//
