LOCUS       sequence-141361-         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid expressing mic-his tag phosphorylation-deficient hHSF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Budzynski MA, Puustinen MC, Joutsen J, Sistonen L
  TITLE     Uncoupling Stress-Inducible Phosphorylation of Heat Shock Factor 1 
            from Its Activation.
  JOURNAL   Mol Cell Biol. 2015 Jul;35(14):2530-40. doi: 10.1128/MCB.00816-14. 
            Epub 2015 May 11.
  PUBMED    25963659
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00816-14"; journalName: "Mol Cell Biol"; date: 
            "2015-07"; volume: "35"; issue: "14"; pages: "2530-40"
COMMENT     Sequence Label: pcDNA-HSF1-Delta∼PRD
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..43)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(59..88)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(101..472)
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
ORIGIN
        1 ttaagctgga gaccgtttaa actcaatgat gatgatgatg atggtcgacg gcgctattca
       61 gatcctcttc tgagatgagt ttttgttcgg gcccaagctt ggagacagtg gggtccttgg
      121 ctttgggagg ctccgagcct gtcagcaggg agatggtggg gtcctcggcg aagccgtccc
      181 cttcggagaa gtaggagccc tctcccagct caaacagcac cggcaggtcg ttgctcccgg
      241 tgtccacgga gccggggtcc agcaggaaca gcggctgcgc tgtgtagtgc accagctgct
      301 tccctgaatc cgggctgctg ttctctgcct cgggaggcct ggggggctcc tggggagaca
      361 ggagctcttg gatactggcc aggctgctgt caaggtcagg caggctcatg tcgggcacgg
      421 tcaccgaggg gctgaacagg tccagcaggg cactggtgtc cacgctgaag ccgtggctgc
      481 tcagcatggt ctgcaggtta tccaggttgg agtccatagc atccaagtgg tcactgagct
      541 cattcttgtc caggcaggct acgctgaggc acttttcagg ggcggcggcg ggcgggggtg
      601 cgggaggccg gccctcggtg tccgtgtggc ccctggcgtc cgtgagggct gtgacggcgg
      661 cgggggcagg ttcagcctcc cgcaggatgg agtcaatgag ggcggtcggg gccaagaggg
      721 cgtccacggc agctgggcgc ccgggagccg cctcctctac ccggggggcc tgaggcgggg
      781 cggggggctc ctccttgaca cgcaccaggg gggcgctgga taggggcctc tcgtctatgc
      841 tcccgccggg ggaggccatg gggctggcag gagccagctc ggtgatgtcg gagatgatgg
      901 gtccagagct ggccacagca tcaggggcgt agaggctgga gctgctgt
//
