LOCUS       sequence-141363-       10758 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes for the 5X repeat of the QUAS response element, followed by 
            the delta pes-10 minimal promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10758)
  AUTHORS   Monsalve GC, Yamamoto KR, Ward JD
  TITLE     A New Tool for Inducible Gene Expression in Caenorhabditis elegans.
  JOURNAL   Genetics. 2019 Feb;211(2):419-430. doi: 10.1534/genetics.118.301705.
            Epub 2018 Nov 30.
  PUBMED    30504365
REFERENCE   2  (bases 1 to 10758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.118.301705"; journalName: "Genetics"; date: 
            "2019-02"; volume: "211"; issue: "2"; pages: "419-430"
COMMENT     Sequence Label: pGM34DEST
FEATURES             Location/Qualifiers
     source          1..10758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1491..1502)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    4106..4230
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4255..4260,4261..4278,4279..4285)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4339..4998
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4405..4424)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5340..5645
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5524..5543
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5686..5810)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(8803..8821)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8889..8993
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8994..9062,9063..9854)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9212..9231)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10025..10613
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10514..10533
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggccctgtc ggattatggg ctcttctttt atcctctact ttctttctag aaaatgcaaa
       61 tgttgagtca gaaattgctg aatggagtac aactgcaatg ggtttagctt atgtttctgg
      121 aacaattact ggtggaattg tgattgaaaa gtaaggttat atttcatttc aataaatgtt
      181 accacagtat aacaaaaaca agttacattg ttcagagtat gtctgaatgt attattatta
      241 ttgtatttgt gcatcagtac tgtagttgta ctgaagaagg gtttaaaata atctcaacgt
      301 ttttaagacg agttagtgag aatacggtcg cagttaggat gtttggacat agttttatta
      361 gataatcgtg aactaactca ctataaaaac ccgctaactc attgaataag atagttttaa
      421 acgttcaaaa tttgaagaca aaattgtatg aataagttta cagagttggt cgacgcaagc
      481 ttctccttct cttcacattt ctcaataatt tagctctaat tgcattcgta ttcttcgcaa
      541 aaatacgtat tctgattgac ccgatgaaat acggatgcct agttgcattg atcatctacg
      601 gttatactta tgggtgaggt ttttcaaata tattttgatt taatcgaata atgaaatttc
      661 agaactggag ttggtccaat ttcgtggttt atatcctctg aactggtacc tcaaaaacat
      721 cgtagtattg ctcaatctgt ggcatattca atgaatactg taatggtggt tatcagtaca
      781 tttacagtat tacctctata ttccgttatt ggtagttatg ctttccttat tctatattca
      841 attccttcat ttataagtat gcttatatta tttcgctact taccggaaac caaaggacga
      901 gagattcatg atattgtgaa tgaacttaaa aaaaaatgaa aacctttgaa aacgttcata
      961 cggccgaaat tttacttgat cgagccaatt gaacttttgt taagacttac agaaaagtga
     1021 gagattttgt tacaaaaaag tgagaaaaaa gcgaaaatga acaggaaaaa gaagaaaaat
     1081 acacccgaag tatcatataa aaattaaaaa gattgtttga cctggcggaa cttttttgca
     1141 aaaactctga atatccatgg cacatgcttc gtgcaaaaca ggatccaaat gaatatcaac
     1201 aagagattct tgaagacgac gagccacttg ctcagagcat tgcttatctc gaatgagacc
     1261 cttgttaaaa tcatttttca aacattatat gacattgtcg aaatgtcctc ctgattccat
     1321 gatggtagca aactcacttc gtctttgagc caatttatcc aagtccttgt aaaaagtatt
     1381 cgaaaattgt taacggataa aatgtttatt ataatatcaa aaacaatttg tcagttgacc
     1441 actttttgat atatttgaca gaaaccggga tgaattggct caaagttagg gcgcccttct
     1501 attacagggt ttctgataac aaaccggtta ttaactccca acaagggatg atttcaattc
     1561 atcatgctca aattgaccca aattaagtta catgacaaat tcatcgccct tttcaactct
     1621 tcttggctca tcatctgtta ttctgttcct attctgtctg caccctatac ccctttgcat
     1681 actctctcgt cattcctttc ttggatagtg tcttcttctc cccagctctg ctacttctat
     1741 gacttgcgcc cgctgctttt ccgctcgttc ctctcctctc tgacgtcttc ttctgcttct
     1801 tttctccttc ctttttccct tctcaatctt cctctttttc ccatttctgt caatcattcg
     1861 aagaagaaga agaagaagac cctcattcat ttcatttttt tttctgtcgg tgtgtgctgc
     1921 tcggttaaga gtgagctctc tattccacgt cttcttcttt ttcttcttcg attcgaatca
     1981 atcactccac caaaacgcat tcgtttttgg gattcacccc gcggttcgca ataggtttct
     2041 ttttcaaatt attagcgtta taaatagaaa aatggtggag tttcaataaa aatgataatt
     2101 tacaaagtgt attttgatta catgtactca aaaaggttga aatttttcat accagttttc
     2161 cggaaatcca tctgatatca ttatcgtatt tttcttttta aaaatgtttt caaaaaaaaa
     2221 caaaatatag ctggttattt ggcaccctct aattaccatt ttcctgtcac accacactct
     2281 tttccttctt ccacttcttt ccgttttcag ccgcttccaa ccaaaccgat atgaaagccg
     2341 agcaacaacg atcgattcca cccggctcgg cgaccttccc gtcgcaggtg agactcagaa
     2401 aactagagaa accgctcaac taactcttga tatccgattt cattcttttc tcttttcttt
     2461 ttgttgaact tttccatatt tccagatgcc acggccacca ccaagcaccg aacaaggaat
     2521 cacaacggaa tcggagcttg cgaagaaagc tcaaatcact ccgaacgacg ttttagcact
     2581 tccgggaatc actcaaggta tcttgctccc tttggatttc agtttaaaac ataaatttag
     2641 gattcttatg ctccccatct gcgaacatct ataacatcga gttcaccaag ttccaaatcc
     2701 gtgatctgga cactgagcaa gtgcttttcg agatcgccaa accggagaac gatcaggaga
     2761 atgatgagtc gccacaggag tcggcaagat acgtgcgtta tagatttgct ccaaactttt
     2821 tgaaactcaa aacggtcgga gcaactgtgg aattcaaagt aggagacatc ccaatccatc
     2881 atttccgaat gatcgaacgt cacttcttca aagatcgcct tctgaagtgt tttgactttg
     2941 aattcggatt ctgtattccg aattcacgaa acaactgtga acatatctat gagttccctc
     3001 aactctctca acaactcagt gagtcattat tctaaaaagt acaattcaaa agactaatct
     3061 ctttttcagt ggacgacatg atcaacaatc caaacgagac tcgttctgac agcttctatt
     3121 tcgtcgataa caaactcgtc atgcacaaca aagccgacta ctcatatgat gcataaatat
     3181 ttaatacaaa aatgttctgg ataattattc tgtcgaatag aaaaaaaact ccaaatgtga
     3241 ttaaattcca ataattcctg tctagtttgt tccttcccct tccccttctc atgttcaatg
     3301 cattcctaag cttttcagtt ccccccttgt ttctatattt tttcgctgtc ctgtcacact
     3361 cgctaaaaac actaatcaca cggaaatctg ttttcaataa aaactccaac tttaactcat
     3421 tttcaatttc aactgaaaga ttttttcatt agagaatgtc tagaactagg cccgggctac
     3481 gtaatacgac tcacttaagg ccttgactag agggtaccag agctcaccta ggtatctaga
     3541 accggtgacg tcaccatggg aagcttcgtg gatccagata tcctgcagga attcctcgag
     3601 acgtacggtg cgcgcgatgc attcgaagat ctgcccacta gggcatgcgg atccgggtaa
     3661 tcgcttatcc tcggataaac aattatcctc acgggtaatc gcttatccgc tcgggtaatc
     3721 gcttatcctc gggtaatcgc ttatccttaa gcttgatatc cctgcaggat cgattttttg
     3781 caaattacga gcgttgtagg gggcggagcg ataggtccta taggttttgg tatatcatca
     3841 ttcattcatt cattggtaca ttcatttacc caccttcctc tttctgagct tctctggagt
     3901 tctgtgcttc ctttttccct tatctttata ctgtaatttt taactttcag ggcgcgcctc
     3961 tagaggatcc ccgggattgg ccaaaggacc caaaggtatg tttcgaatga tactaacata
     4021 acatagaaca ttttcaggag gacccttggc tagcgtcgac ggtaccggta gaaaaatcca
     4081 gtgtggtgga attctgcaga tatcaacaag tttgtacaaa aaagctgaac gagaaacgta
     4141 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
     4201 ctgtaaaaca caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca
     4261 ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccggc gagattttca
     4321 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     4381 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
     4441 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     4501 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt
     4561 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     4621 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     4681 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     4741 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     4801 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     4861 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     4921 gtctgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     4981 tggcagggcg gggcgtaaag atctggatcc ggcttactaa aagccagata acagtatgcg
     5041 tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg
     5101 tcaaaaagag gtgtgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc
     5161 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa
     5221 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga
     5281 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg actggtgaaa
     5341 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac
     5401 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc
     5461 tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct
     5521 ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg
     5581 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa
     5641 tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata
     5701 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
     5761 atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt tgatatccag
     5821 cacagtggcg gccgctcgac attcgtagaa ttccaactga gcgccggtcg ctaccattac
     5881 caacttgtct ggtgtcaaaa ataatagggg ccgctgtcat cagagtaagt ttaaactgag
     5941 ttctactaac taacgagtaa tatttaaatt ttcagcatct cgcgcccgtg cctctgactt
     6001 ctaagtccaa ttactcttca acatccctac atgctctttc tccctgtgct cccaccccct
     6061 atttttgtta ttatcaaaaa acttctctta atttctttgt tttttagctt cttttaagtc
     6121 acctctaaca atgaaattgt gtagattcaa aaatagaatt aattcgtaat aaaaagtcga
     6181 aaaaaattgt gctccctccc cccattaata ataattctat cccaaaatct acacaatgtt
     6241 ctgtgtacac ttcttatgtt ttttacttct gataaatttt tttgaaacat catagaaaaa
     6301 accgcacaca aaatacctta tcatatgtta cgtttcagtt tatgaccgca atttttattt
     6361 cttcgcacgt ctgggcctct catgacgtca aatcatgctc atcgtgaaaa agttttggag
     6421 tatttttgga atttttcaat caagtgaaag tttatgaaat taattttcct gcttttgctt
     6481 tttgggggtt tcccctattg tttgtcaaga gtttcgagga cggcgttttt cttgctaaaa
     6541 tcacaagtat tgatgagcac gatgcaagaa agatcggaag aaggtttggg tttgaggctc
     6601 agtggaaggt gagtagaagt tgataatttg aaagtggagt agtgtctatg gggtttttgc
     6661 cttaaatgac agaatacatt cccaatatac caaacataac tgtttcctac tagtcggccg
     6721 tacgggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     6781 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     6841 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     6901 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     6961 taccgcatca ggcggcctta attaatctag tgagtcgtat tataagtgca agtaagatca
     7021 gtgtttgttt cgatatcagt ctgtttcgta acggtcttct gtataactac aaaaaagaat
     7081 aaaaaaccgt atcacaactg agaaaaaaga caaaacttat tgaacagtgt tcctttcaaa
     7141 aaattatttt gtaaaaaatc gagtttttct ccaaaataac gcacgcaatc aaaaacaaga
     7201 aaactaacgt gttctcctgg gcgtggaact ccttatcaag attaccatta ttgtctgtca
     7261 gaattgatat ttcaattatt tcatatctag acaaacccgg gtgtcttgca tttattagta
     7321 ctgtgagttt acatacaaac gaggcttact cagataacat agagtcacct ctaattagca
     7381 aaaacccatt tctaattagc tatttcgacc agttctaaag tcaccgtaat tagaaatggt
     7441 ttttgtagct tttctaatta gaaatgaccc cgagataagt gaaatatggc attttctggg
     7501 ttttctagta agaaatgatc ccgcacgtag tgattccgcg ttacaagcca aattcaaatt
     7561 aaaaaagtat attacacaat tcaagttaca ttattttcag gaaacaatgg cgaagagcaa
     7621 agcagagatt caaaaggagg aaagagacaa cagatatgaa cgaagacttc ttgaaaagag
     7681 aaagagacat gaggtgagta accatgataa taaataatca agtaagggct atagatttat
     7741 aaattctgga gttaacagtt tcgaaaaaaa gtagttttca tatgtgcgaa ggaatatcaa
     7801 aaatagggga atagcctaaa cgagtgagaa aattaaatgt ttgttatttt ttcaaaaatg
     7861 tctgcagggc cgttatcctt tcaaatgcac tctatcaaac atagatcata tccatcatat
     7921 cttatccaga atttatatca tatccaagat tgagagcgtt tcaaggaaaa tctcaaacaa
     7981 ccatacaatt ttgtagatcg acaacaaggc ctccacttct tcatcgatgg cctctgcagc
     8041 acctgcacca ccaagtttgt atttttcata ttaatataaa gtgagccacg catagtcgtt
     8101 tttaggattt ttgccagatc gatgtcgcgc aaacagactg tttttttttc tttgaaagcc
     8161 gtgcgtgaca tgggccacgg attgtaacgc cgcgctggat ggataaaaat ttattcagtt
     8221 ttttagcctt tacaaaatat ggttcgacta ctttctttat ttttacattt ttataggtgc
     8281 tcattgagct gagatatttt gggtataatc ggaaaatcgc tgattttggt catttttctg
     8341 cactaaagat tacacaaaaa gttcagtatt tagattcatt gttgaaaatt ggggatgatc
     8401 tacagataca tatcgccgtg ttcaaaaaca cacgtgctgg cgttacccaa cttaatcgcc
     8461 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     8521 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
     8581 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     8641 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     8701 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     8761 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     8821 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     8881 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     8941 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     9001 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     9061 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     9121 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     9181 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     9241 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     9301 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     9361 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     9421 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     9481 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     9541 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     9601 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     9661 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     9721 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     9781 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     9841 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     9901 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     9961 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    10021 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    10081 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    10141 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
    10201 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    10261 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    10321 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    10381 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    10441 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    10501 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    10561 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    10621 gcaacgcggc ctttttacgg ttcctggtct tttgctggcc ttttgctcac atgttctttc
    10681 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgatacca
    10741 gcggataaca atttcaca
//
