LOCUS       sequence-141370-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 2 Destination vector - Position D - Assembly of 4-5 TU's from 
            Level 1-ABCD or ABCDE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pTU2-D-RFP
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(41..98)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(133..160)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(176..247)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gcgagtcagt gagcgaggaa gcctgcataa cgcgaagtaa tcttttcggt tttaaagaaa
       61 aagggcaggg tggtgacacc ttgccctttt ttgccggact gcagggtctc taacactaat
      121 gagacgtata aacgcagaaa ggcccacccg aaggtgagcc agtgtgactc tagtagagag
      181 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
      241 ttatttgatg cctggctcta gtagcgatct acactagcac tatcagcgtt attaagcacc
      301 ggtggagtga cgaccttcag cacgttcgta ctgttcaacg atggtgtagt cttcgttgtg
      361 ggaggtgatg tccagtttga tgtcggtttt gtaagcaccc ggcagctgaa ccggtttttt
      421 agccatgtag gtggttttaa cttcagcgtc gtagtgacca ccgtctttca gtttcagacg
      481 cattttgatt tcacctttca gagcaccgtc ttccgggtac atacgttcgg tggaagcttc
      541 ccaacccatg gtttttttct gcataaccgg accgtcggac gggaagttgg taccacgcag
      601 tttaactttg tagatgaact caccgtcttg cagggaggag tcctgggtaa cggtaacaac
      661 accaccgtct tcgaagttca taacacgttc ccatttgaaa ccttccggga aggacagttt
      721 caggtagtcc gggatgtcag ccgggtgttt aacgtaagct ttggaaccgt actggaactg
      781 cggggacagg atgtcccaag cgaacggcag cggaccacct ttggtaactt tcagtttagc
      841 ggtctgggta ccttcgtacg gacgac
//
