LOCUS       sequence-141371-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 2 Destination vector - Position C - Assembly of 4-5 TU's from 
            Level 1-ABCD or ABCDE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pTU2-C-RFP
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..20
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(44..101)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(136..163)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(179..250)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gcagcgagtc agtgagcgag gaagcctgca taacgcgaag taatcttttc ggttttaaag
       61 aaaaagggca gggtggtgac accttgccct tttttgccgg actgcagggt ctcttcgact
      121 aatgagacgt ataaacgcag aaaggcccac ccgaaggtga gccagtgtga ctctagtaga
      181 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc
      241 gttttatttg atgcctggct ctagtagcga tctacactag cactatcagc gttattaagc
      301 accggtggag tgacgacctt cagcacgttc gtactgttca acgatggtgt agtcttcgtt
      361 gtgggaggtg atgtccagtt tgatgtcggt tttgtaagca cccggcagct gaaccggttt
      421 tttagccatg taggtggttt taacttcagc gtcgtagtga ccaccgtctt tcagtttcag
      481 acgcattttg atttcacctt tcagagcacc gtcttccggg tacatacgtt cggtggaagc
      541 ttcccaaccc atggtttttt tctgcataac cggaccgtcg gacgggaagt tggtaccacg
      601 cagtttaact ttgtagatga actcaccgtc ttgcagggag gagtcctggg taacggtaac
      661 aacaccaccg tcttcgaagt tcataacacg ttcccatttg aaaccttccg ggaaggacag
      721 tttcaggtag tccgggatgt cagccgggtg tttaacgtaa gctttggaac cgtactggaa
      781 ctgcggggac aggatgtccc aagcgaacgg cagcggacca cctttggtaa ctttcagttt
      841 agcggtctgg gtaccttcgt acggacgacc t
//
