LOCUS       sequence-141372-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 2 Destination vector - Position B - Assembly of 4-5 TU's from 
            Level 1-ABCD or ABCDE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pTU2-B-RFP
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(40..97)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(132..159)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(175..246)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgagtcagtg agcgaggaag cctgcataac gcgaagtaat cttttcggtt ttaaagaaaa
       61 agggcagggt ggtgacacct tgcccttttt tgccggactg cagggtctct gtccctaatg
      121 agacgtataa acgcagaaag gcccacccga aggtgagcca gtgtgactct agtagagagc
      181 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt
      241 tatttgatgc ctggctctag tagcgatcta cactagcact atcagcgtta ttaagcaccg
      301 gtggagtgac gaccttcagc acgttcgtac tgttcaacga tggtgtagtc ttcgttgtgg
      361 gaggtgatgt ccagtttgat gtcggttttg taagcacccg gcagctgaac cggtttttta
      421 gccatgtagg tggttttaac ttcagcgtcg tagtgaccac cgtctttcag tttcagacgc
      481 attttgattt cacctttcag agcaccgtct tccgggtaca tacgttcggt ggaagcttcc
      541 caacccatgg tttttttctg cataaccgga ccgtcggacg ggaagttggt accacgcagt
      601 ttaactttgt agatgaactc accgtcttgc agggaggagt cctgggtaac ggtaacaaca
      661 ccaccgtctt cgaagttcat aacacgttcc catttgaaac cttccgggaa ggacagtttc
      721 aggtagtccg ggatgtcagc cgggtgttta acgtaagctt tggaaccgta ctggaactgc
      781 ggggacagga tgtcccaagc gaacggcagc ggaccacctt tggtaacttt cagtttagcg
      841 gtctgggtac cttcgtacgg acgaccttca ccttcac
//
