LOCUS       sequence-141374-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 2 Destination vector - Position B - Assembly of 3 TU's from 
            Level 1-ABC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pTU2-b-RFP
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..19
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(43..100)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(135..162)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(178..249)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cagcgagtca gtgagcgagg aagcctgcat aacgcgaagt aatcttttcg gttttaaaga
       61 aaaagggcag ggtggtgaca ccttgccctt ttttgccgga ctgcagggtc tctgtacctt
      121 ctgagacgta taaacgcaga aaggcccacc cgaaggtgag ccagtgtgac tctagtagag
      181 agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg
      241 ttttatttga tgcctggctc tagtagcgat ctacactagc actatcagcg ttattaagca
      301 ccggtggagt gacgaccttc agcacgttcg tactgttcaa cgatggtgta gtcttcgttg
      361 tgggaggtga tgtccagttt gatgtcggtt ttgtaagcac ccggcagctg aaccggtttt
      421 ttagccatgt aggtggtttt aacttcagcg tcgtagtgac caccgtcttt cagtttcaga
      481 cgcattttga tttcaccttt cagagcaccg tcttccgggt acatacgttc ggtggaagct
      541 tcccaaccca tggttttttt ctgcataacc ggaccgtcgg acgggaagtt ggtaccacgc
      601 agtttaactt tgtagatgaa ctcaccgtct tgcagggagg agtcctgggt aacggtaaca
      661 acaccaccgt cttcgaagtt cataacacgt tcccatttga aaccttccgg gaaggacagt
      721 ttcaggtagt ccgggatgtc agccgggtgt ttaacgtaag ctttggaacc gtactggaac
      781 tgcggggaca ggatgtccca agcgaacggc agcggaccac ctttggtaac tttcagttta
      841 gcggtctggg tac
//
