LOCUS       sequence-141375-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Entry vector"" ORF - eGFP                         "".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-ORF-eGFP
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..20
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(44..101)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(178..198)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     793..821
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gcagcgagtc agtgagcgag gaagcctgca taacgcgaag taatcttttc ggttttaaag
       61 aaaaagggca gggtggtgac accttgccct tttttgccgg actgcagcta atgagacggc
      121 atgggtctct tcgattattt gtatagttca tccatgccat gtgtaatccc agcagctgtt
      181 acaaactcaa gaaggaccat gtgatctctc ttttcgttgg gatctttcga aagggcagat
      241 tgtgtggaca ggtaatggtt gtctggtaaa aggacagggc catcgccaat tggagtattt
      301 tgttgataat ggtctgctag ttgaacgctt ccatcttcaa tgttgtgtct aattttgaag
      361 ttaactttga ttccattctt ttgtttgtct gccatgatgt atacattgtg tgagttatag
      421 ttgtattcca atttgtgtcc aagaatgttt ccatcttctt taaaatcaat accttttaac
      481 tcgattctat taacaagggt atcaccttca aacttgactt cagcacgtgt cttgtagttc
      541 ccgtcatctt tgaaaaatat agttctttcc tgtacataac cttcgggcat ggcactcttg
      601 aaaaagtcat gctgtttcat atgatctggg tatctcgcaa agcattgaac accataaccg
      661 aaagtagtga caagtgttgg ccatggaaca ggtagttttc cagtagtgca aataaattta
      721 agggtaagtt ttccgtatgt tgcatcacct tcaccctctc cactgacaga aaatttgtgc
      781 ccattaacat caccatctaa ttcaacaaga attgggacaa ctccagtgaa aagttcttct
      841 c
//
