LOCUS       sequence-141376-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Entry vector"" ORF - mCherry                            "".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-ORF-mCherry
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..20
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(44..101)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     CDS             complement(141..851)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(419..438)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     688..706
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gcagcgagtc agtgagcgag gaagcctgca taacgcgaag taatcttttc ggttttaaag
       61 aaaaagggca gggtggtgac accttgccct tttttgccgg actgcagcta atgagacggc
      121 atgggtctct tcgaggatcc ttacttgtac agctcgtcca tgccgccggt ggagtggcgg
      181 ccctcggcgc gttcgtactg ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc
      241 aacttgatgt tgacgttgta ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg
      301 gtcttgacct cagcgtcgta gtggccgccg tccttcagct tcagcctctg cttgatctcg
      361 cccttcaggg cgccgtcctc ggggtacatc cgctcggagg aggcctccca gcccatggtc
      421 ttcttctgca ttacggggcc gtcggagggg aagttggtgc cgcgcagctt caccttgtag
      481 atgaactcgc cgtcctgcaa ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg
      541 aagttcatca cgcgctccca cttgaagccc tcggggaagg acagcttcaa gtagtcgggg
      601 atgtcggcgg ggtgcttcac gtaggccttg gagccgtaca tgaactgagg ggacaggatg
      661 tcccaggcga agggcagggg gccacccttg gtcaccttca gcttggcggt ctgggtgccc
      721 tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggagccc
      781 tccatgtgca ccttgaagcg catgaactcc ttgatgatgg ccatgttatc ctcctcgccc
      841 ttgctcacca tatgtgagac ctatgcgtct ctagat
//
