LOCUS       sequence-141377-         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Entry vector"" ORF - eCFP"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-ORF-eCFP
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..20
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(44..101)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(153..174)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(309..821,822..824,825..827))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     502..521
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     761..782
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    854..873
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 gcagcgagtc agtgagcgag gaagcctgca taacgcgaag taatcttttc ggttttaaag
       61 aaaaagggca gggtggtgac accttgccct tttttgccgg actgcagcta atgagacggc
      121 atgggtctct tcgaggatcc ttaggcggcg gtcacgaact ccagcaggac catgtgatcg
      181 cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc
      241 agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg
      301 ctgccgtcct cgatgttgtg gcggatcttg aagttggcct tgatgccgtt cttctgcttg
      361 tcggcggtga tatagacgtt gtggctgatg tagttgtact ccagcttgtg ccccaggatg
      421 ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc
      481 tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc
      541 tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg
      601 gggtagcggc tgaagcactg cacgccccag gtcagggtgg tcacgagggt gggccagggc
      661 acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg
      721 ccctcgccct cgccggacac gctgaacttg tggccgttca cgtcgccgtc cagctcgacc
      781 aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatatg tgagacctat
      841 gcgtctctag attctagaag cggccgcgaa ttccagaa
//
