LOCUS       sequence-141378-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 - J23108 standard iGEM promoter (5' CTAT/3' GTAC fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-J23108
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..20
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(44..101)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     promoter        complement(135..169)
                     /label=BBa_J23108
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.51"
     misc_feature    197..216
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      219..262
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(266..284)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(512..531)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gcagcgagtc agtgagcgag gaagcctgca taacgcgaag taatcttttc ggttttaaag
       61 aaaaagggca gggtggtgac accttgccct tttttgccgg actgcagcta atgagacggc
      121 atgggtctct gtacgctagc attataccta ggactgagct agctgtcaga tagtgagacc
      181 tatgcgtctc tagattctag aagcggccgc gaattccaga aatcatcctt agcgaaagct
      241 aaggattttt tttatctgaa attctgcctc gtgatacgcc tatttttata ggttaatgtc
      301 atgataataa tggtttctta gacgtcaggt ggcactcgag ttgatcgggc acgtaagagg
      361 ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg agttatcgag
      421 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga
      481 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac
      541 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa
      601 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga
      661 atttcgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta
      721 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga
      781 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aa
//
