LOCUS       sequence-141379-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 - J23114 standard iGEM promoter (5' CTAT/3' GTAC fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-J23114
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(40..97)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     promoter        complement(131..165)
                     /label=BBa_J23114
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.1"
     misc_feature    193..212
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      215..258
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(262..280)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(508..527)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgagtcagtg agcgaggaag cctgcataac gcgaagtaat cttttcggtt ttaaagaaaa
       61 agggcagggt ggtgacacct tgcccttttt tgccggactg cagctaatga gacggcatgg
      121 gtctctgtac gctagcattg tacctaggac tgagctagcc ataaaatagt gagacctatg
      181 cgtctctaga ttctagaagc ggccgcgaat tccagaaatc atccttagcg aaagctaagg
      241 atttttttta tctgaaattc tgcctcgtga tacgcctatt tttataggtt aatgtcatga
      301 taataatggt ttcttagacg tcaggtggca ctcgagttga tcgggcacgt aagaggttcc
      361 aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt
      421 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
      481 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
      541 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
      601 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattt
      661 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
      721 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
      781 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaa
//
