LOCUS       sequence-141380-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 - SJM914 promoter (5' CTAT/3' GTAC fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-SJM914
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..19
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(43..100)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(128..155)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(171..242)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(298..318)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cagcgagtca gtgagcgagg aagcctgcat aacgcgaagt aatcttttcg gttttaaaga
       61 aaaagggcag ggtggtgaca ccttgccctt ttttgccgga ctgcagcgtc tctggcaaac
      121 atataaacgc agaaaggccc acccgaaggt gagccagtgt gactctagta gagagcgttc
      181 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
      241 tgatgcctgg tcgattattt gtatagttca tccatgccat gtgtaatccc agcagctgtt
      301 acaaactcaa gaaggaccat gtgatctctc ttttcgttgg gatctttcga aagggcagat
      361 tgtgtggaca ggtaatggtt gtctggtaaa aggacagggc catcgccaat tggagtattt
      421 tgttgataat ggtctgctag ttgaacgctt ccatcttcaa tgttgtgtct aattttgaag
      481 ttaactttga ttccattctt ttgtttgtct gccatgatgt atacattgtg tgagttatag
      541 ttgtattcca atttgtgtcc aagaatgttt ccatcttctt taaaatcaat accttttaac
      601 tcgattctat taacaagggt atcaccttca aacttgactt cagcacgtgt cttgtagttc
      661 ccgtcatctt tgaaaaatat agttctttcc tgtacataac cttcgggcat ggcactcttg
      721 aaaaagtcat gctgtttcat atgatctggg tatctcgcaa agcattgaac accataaccg
      781 aaagtagtga caagtgttgg ccatggaaca ggtagtt
//
