LOCUS       sequence-141381-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 - SJM912 promoter (5' CTAT/3' GTAC fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-SJM912
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(41..98)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(126..153)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(169..240)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(296..316)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gcgagtcagt gagcgaggaa gcctgcataa cgcgaagtaa tcttttcggt tttaaagaaa
       61 aagggcaggg tggtgacacc ttgccctttt ttgccggact gcagcgtctc tggcaaacat
      121 ataaacgcag aaaggcccac ccgaaggtga gccagtgtga ctctagtaga gagcgttcac
      181 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg
      241 atgcctggtc gattatttgt atagttcatc catgccatgt gtaatcccag cagctgttac
      301 aaactcaaga aggaccatgt gatctctctt ttcgttggga tctttcgaaa gggcagattg
      361 tgtggacagg taatggttgt ctggtaaaag gacagggcca tcgccaattg gagtattttg
      421 ttgataatgg tctgctagtt gaacgcttcc atcttcaatg ttgtgtctaa ttttgaagtt
      481 aactttgatt ccattctttt gtttgtctgc catgatgtat acattgtgtg agttatagtt
      541 gtattccaat ttgtgtccaa gaatgtttcc atcttcttta aaatcaatac cttttaactc
      601 gattctatta acaagggtat caccttcaaa cttgacttca gcacgtgtct tgtagttccc
      661 gtcatctttg aaaaatatag ttctttcctg tacataacct tcgggcatgg cactcttgaa
      721 aaagtcatgc tgtttcatat gatctgggta tctcgcaaag cattgaacac cataaccgaa
      781 agtagtgaca agtgttggcc atggaacagg tagttttcca gtagtgcaaa taa
//
