LOCUS       sequence-141386-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 - SJM908 promoter (5' CTAT/3' GTAC fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-SJM908
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..20
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(44..101)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(129..156)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(172..243)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(299..319)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gcagcgagtc agtgagcgag gaagcctgca taacgcgaag taatcttttc ggttttaaag
       61 aaaaagggca gggtggtgac accttgccct tttttgccgg actgcagcgt ctctggcaaa
      121 catataaacg cagaaaggcc cacccgaagg tgagccagtg tgactctagt agagagcgtt
      181 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
      241 ttgatgcctg gtcgattatt tgtatagttc atccatgcca tgtgtaatcc cagcagctgt
      301 tacaaactca agaaggacca tgtgatctct cttttcgttg ggatctttcg aaagggcaga
      361 ttgtgtggac aggtaatggt tgtctggtaa aaggacaggg ccatcgccaa ttggagtatt
      421 ttgttgataa tggtctgcta gttgaacgct tccatcttca atgttgtgtc taattttgaa
      481 gttaactttg attccattct tttgtttgtc tgccatgatg tatacattgt gtgagttata
      541 gttgtattcc aatttgtgtc caagaatgtt tccatcttct ttaaaatcaa taccttttaa
      601 ctcgattcta ttaacaaggg tatcaccttc aaacttgact tcagcacgtg tcttgtagtt
      661 cccgtcatct ttgaaaaata tagttctttc ctgtacataa ccttcgggca tggcactctt
      721 gaaaaagtca tgctgtttca tatgatctgg gtatctcgca aagcattgaa caccataacc
      781 gaaagtagtg acaagtgttg gccatggaac aggtagtttt ccagtagtgc aaataaattt
      841 a
//
