LOCUS       sequence-141392-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Hexahistidine N-terminal tag (5' TAAA/3' CATA fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-HexHis
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(30..87)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     CDS             complement(118..135)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    165..184
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      187..230
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(234..252)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(480..499)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agcgaggaag cctgcataac gcgaagtaat cttttcggtt ttaaagaaaa agggcagggt
       61 ggtgacacct tgcccttttt tgccggactg cagctaatga gacggcatgg gtctcttatg
      121 gtgatggtga tggtgcattt atgagaccta tgcgtctcta gattctagaa gcggccgcga
      181 attccagaaa tcatccttag cgaaagctaa ggattttttt tatctgaaat tctgcctcgt
      241 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
      301 cactcgagtt gatcgggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac
      361 taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga
      421 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
      481 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
      541 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
      601 ttgcccgcct gatgaatgct catccggaat ttcgtatggc aatgaaagac ggtgagctgg
      661 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
      721 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
      781 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gaga
//
