LOCUS       sequence-141393-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal linker with pET-RBS (5' GTAC/3' TAAA fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-Tag_linker
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(36..93)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    177..196
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      199..242
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(246..264)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(492..511)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcagtgagcg aggaagcctg cataacgcga agtaatcttt tcggttttaa agaaaaaggg
       61 cagggtggtg acaccttgcc cttttttgcc ggactgcagc taatgagacg gcatgggtct
      121 cttttatata tctccttctt aaagttaaag tactgagacc tatgcgtctc tagattctag
      181 aagcggccgc gaattccaga aatcatcctt agcgaaagct aaggattttt tttatctgaa
      241 attctgcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
      301 gacgtcaggt ggcactcgag ttgatcgggc acgtaagagg ttccaacttt caccataatg
      361 aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag
      421 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
      481 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
      541 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
      601 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag
      661 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
      721 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
      781 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagg
//
