LOCUS       sequence-141394-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Secondary module (BpiI) site in pTU2a backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pTU2_2a
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(36..93)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    146..167
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     primer_bind     complement(398..417)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcagtgagcg aggaagcctg cataacgcga agtaatcttt tcggttttaa agaaaaaggg
       61 cagggtggtg acaccttgcc cttttttgcc ggactgcagg gtctctgtac ccggtgagac
      121 ggtgcgtctc tagatatagt gagacctcta gaagcggccg cgaattcgta cttgtcttcg
      181 catgcgtgca tatggaagac ttataggacg tcaggtggca ctcgagttga tcgggcacgt
      241 aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt
      301 atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
      361 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
      421 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
      481 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
      541 tccggaattt cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
      601 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
      661 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
      721 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtgtcag ccaatccctg
      781 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
      841 tttcaccatg ggcaa
//
