LOCUS       sequence-141396-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  L2U3H03 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-L2U3H03
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..20
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(44..101)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    202..221
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      224..267
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(271..289)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(517..536)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gcagcgagtc agtgagcgag gaagcctgca taacgcgaag taatcttttc ggttttaaag
       61 aaaaagggca gggtggtgac accttgccct tttttgccgg actgcagcta atgagacggc
      121 atgcggtctc taacactcaa caaatagcgt gaccgatgcg ccggtcacgc tatcgatgag
      181 acccatatgc gtctctagat tctagaagcg gccgcgaatt ccagaaatca tccttagcga
      241 aagctaagga ttttttttat ctgaaattct gcctcgtgat acgcctattt ttataggtta
      301 atgtcatgat aataatggtt tcttagacgt caggtggcac tcgagttgat cgggcacgta
      361 agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta
      421 tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc
      481 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa
      541 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa
      601 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat
      661 ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct
      721 tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tga
//
