LOCUS       sequence-141397-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Secondary module (BpiI) site in pTU2-a backbone with colE1 
            (medium-copy) origin of replication.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pTU2S-a (colE1 origin)
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(10..67)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    120..141
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     primer_bind     complement(372..391)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gcgaagtaat cttttcggtt ttaaagaaaa agggcagggt ggtgacacct tgcccttttt
       61 tgccggactg cagggtctct gtacccggtg agacggtgcg tctctagata tagtgagacc
      121 tctagaagcg gccgcgaatt cgtacttgtc ttcgcatgcg tgcatatgga agacttatag
      181 gacgtcaggt ggcactcgag ttgatcgggc acgtaagagg ttccaacttt caccataatg
      241 aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag
      301 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
      361 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
      421 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
      481 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag
      541 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
      601 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
      661 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
      721 ttgagaatat gtttttcgtg tcagccaatc cctgggtgag tttcaccagt tttgatttaa
      781 acgtggccaa tatggacaac ttcttcgccc c
//
