LOCUS       sequence-141405-         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1 WT 
            RD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Budzynski MA, Puustinen MC, Joutsen J, Sistonen L
  TITLE     Uncoupling Stress-Inducible Phosphorylation of Heat Shock Factor 1 
            from Its Activation.
  JOURNAL   Mol Cell Biol. 2015 Jul;35(14):2530-40. doi: 10.1128/MCB.00816-14. 
            Epub 2015 May 11.
  PUBMED    25963659
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00816-14"; journalName: "Mol Cell Biol"; date: 
            "2015-07"; volume: "35"; issue: "14"; pages: "2530-40"
COMMENT     Sequence Label: Gal4-VP16-HSF1 WT
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             91..531
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaagaggctt ttttggagga
       61 gatctaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat cgaacaagca
      121 tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa gtgcgccaag
      181 tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc tccgctgact
      241 agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct atttctactg
      301 atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca ggatataaaa
      361 gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt cacagataga
      421 ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat aagtgcgaca
      481 tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc gccggaattc
      541 cattccatgc ccaagtatag ccggcagttc tccctggagc acgtccacgg ctcgggcccc
      601 tactcggccc cctccccagc ctacagcagc tccagcctct acgcccctga tgctgtggcc
      661 agctctggac ccatcatctc cgacatcacc gagctggctc ctgccagccc catggcctcc
      721 cccggcggga gcatagacga gaggccccta tccagcagcc ccctggtgcg tgtcaaggag
      781 gagcccccca gcccgcctca gagcccccgg gtagaggagg cgagtcccgg gcgcccatct
      841 tccgtggaca ccctcttgtc cccgaccgcc ctcattgact ccatcctgcg gga
//
