LOCUS       sequence-141406-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1 WT 
            RD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Budzynski MA, Puustinen MC, Joutsen J, Sistonen L
  TITLE     Uncoupling Stress-Inducible Phosphorylation of Heat Shock Factor 1 
            from Its Activation.
  JOURNAL   Mol Cell Biol. 2015 Jul;35(14):2530-40. doi: 10.1128/MCB.00816-14. 
            Epub 2015 May 11.
  PUBMED    25963659
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00816-14"; journalName: "Mol Cell Biol"; date: 
            "2015-07"; volume: "35"; issue: "14"; pages: "2530-40"
COMMENT     Sequence Label: Gal4-VP16-HSF1 WT
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(131..364)
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
ORIGIN
        1 ggaatccacc aaataatcag acgacaaccg caggtttttg taatgtatgt gctcgtgttt
       61 attgtggata cgaaccgggg acgggagggg aaaacccaga cgggggatgc gggtccggtc
      121 gcgcccccta cccaccgtac tcgtcaattc caagggcatc ggtaaacatc tgctcaaact
      181 cgaagtcggc catatccaga gcgccgtagg gggcggagtc gtggggggta aatcccggac
      241 ccggggaatc cccgtccccc aacatgtcca gatcgaaatc gtctagcgcg tcggcatgcg
      301 ccatcgccac gtcctcgccg tctaagtgga gctcgtcccc caggctgaca tcggtcgggg
      361 gggcccagat ccccgggaat tccaggcagg ctacgctgag gcacttttca ggggtggagg
      421 tgggcggggg ggagggaggc cggccctcgg tgtccgtgtg gcccctggcg tccgtgaggg
      481 ctgtgacgga ggcgggggca ggttcactct cccgcaggat ggagtcaatg agggcggtcg
      541 gggacaagag ggtgtccacg gaagatgggc gcccgggact cgcctcctct acccgggggc
      601 tctgaggcgg gctggggggc tcctccttga cacgcaccag ggggctgctg gataggggcc
      661 tctcgtctat gctcccgccg ggggaggcca tggggctggc aggagccagc tcggtgatgt
      721 cggagatgat gggtccagag ctggccacag catcaggggc gtagaggctg gagctgctgt
      781 aggctgggga gggggccgag taggggcccg agccgtggac gtgctccagg gagaactgcc
      841 ggctatactt gggcatggaa tggaattccg gcgatacagt caactgtctt tgacctttgt
      901 tactactctc ttccgatgat gatgtcgc
//
