LOCUS       sequence-141410-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid expressing fusion construct of Gal4 DBD and VP16 AD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Budzynski MA, Puustinen MC, Joutsen J, Sistonen L
  TITLE     Uncoupling Stress-Inducible Phosphorylation of Heat Shock Factor 1 
            from Its Activation.
  JOURNAL   Mol Cell Biol. 2015 Jul;35(14):2530-40. doi: 10.1128/MCB.00816-14. 
            Epub 2015 May 11.
  PUBMED    25963659
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00816-14"; journalName: "Mol Cell Biol"; date: 
            "2015-07"; volume: "35"; issue: "14"; pages: "2530-40"
COMMENT     Sequence Label: Gal4-VP16
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(133..366)
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             complement(388..828)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 cccggatcca ccaaataatc agacgacaac cgcaggtttt tgtaatgtat gtgctcgtgt
       61 ttattgtgga tacgaaccgg ggacgggagg ggaaaaccca gacgggggat gcgggtccgg
      121 tcgcgccccc tacccaccgt actcgtcaat tccaagggca tcggtaaaca tctgctcaaa
      181 ctcgaagtcg gccatatcca gagcgccgta gggggcggag tcgtgggggg taaatcccgg
      241 acccggggaa tccccgtccc ccaacatgtc cagatcgaaa tcgtctagcg cgtcggcatg
      301 cgccatcgcc acgtcctcgc cgtctaagtg gagctcgtcc cccaggctga catcggtcgg
      361 gggggcccag atccccggga attccggcga tacagtcaac tgtctttgac ctttgttact
      421 actctcttcc gatgatgatg tcgcacttat tctatgctgt ctcaatgtta gaggcatatc
      481 agtctccact gaagccaatc tatctgtgac ggcatcttta ttcacattat cttgtacaaa
      541 taatcctgtt aacaatgctt ttatatcctg taaagaatcc attttcaaaa tcatgtcaag
      601 gtcttctcga ggaaaaatca gtagaaatag ctgttccagt ctttctagcc ttgattccac
      661 ttctgtcaga tgtgccctag tcagcggaga ccttttggtt ttgggagagt agcgacactc
      721 ccagttgttc ttcagacact tggcgcactt cggtttttct ttggagcact tgagcttttt
      781 aagtcggcaa atatcgcatg cttgttcgat agaagacagt agcttcatct ttcaggaggc
      841 ttgcttcaag cttagatctc ctccaaaaaa gcctcttcac tacttctgga atagctcaga
      901 ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
      961 atgggcggaa ctgggcggag ttaggggcgg
//
