LOCUS       sequence-141411-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1 WT 
            RD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Budzynski MA, Puustinen MC, Joutsen J, Sistonen L
  TITLE     Uncoupling Stress-Inducible Phosphorylation of Heat Shock Factor 1 
            from Its Activation.
  JOURNAL   Mol Cell Biol. 2015 Jul;35(14):2530-40. doi: 10.1128/MCB.00816-14. 
            Epub 2015 May 11.
  PUBMED    25963659
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00816-14"; journalName: "Mol Cell Biol"; date: 
            "2015-07"; volume: "35"; issue: "14"; pages: "2530-40"
COMMENT     Sequence Label: Gal4-VP16-HSF1 WT
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             507..740
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(892..911)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    936..984
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 cggaattcca ttccatgccc aagtatagcc ggcagttctc cctggagcac gtccacggct
       61 cgggccccta ctcggccccc tccccagcct acagcagctc cagcctctac gcccctgatg
      121 ctgtggccag ctctggaccc atcatctccg acatcaccga gctggctcct gccagcccca
      181 tggcctcccc cggcgggagc atagacgaga ggcccctatc cagcagcccc ctggtgcgtg
      241 tcaaggagga gccccccagc ccgcctcaga gcccccgggt agaggaggcg agtcccgggc
      301 gcccatcttc cgtggacacc ctcttgtccc cgaccgccct cattgactcc atcctgcggg
      361 agagtgaacc tgcccccgcc tccgtcacag ccctcacgga cgccaggggc cacacggaca
      421 ccgagggccg gcctccctcc cccccgccca cctccacccc tgaaaagtgc ctcagcgtag
      481 cctgcctgga attcccgggg atctgggccc ccccgaccga tgtcagcctg ggggacgagc
      541 tccacttaga cggcgaggac gtggcgatgg cgcatgccga cgcgctagac gatttcgatc
      601 tggacatgtt gggggacggg gattccccgg gtccgggatt taccccccac gactccgccc
      661 cctacggcgc tctggatatg gccgacttcg agtttgagca gatgtttacc gatgcccttg
      721 gaattgacga gtacggtggg tagggggcgc gaccggaccc gcatcccccg tctgggtttt
      781 cccctcccgt ccccggttcg tatccacaat aaacacgagc acatacatta caaaaacctg
      841 cggttgtcgt ctgattattt ggtggatccg ggggagatgg gggaggctaa ctgaaacacg
      901 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
      961 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     1021 tcgatacccc accgagaccc cattggggcc aatacgcccg cg
//
