LOCUS       sequence-141412-        4616 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1 WT 
            RD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Budzynski MA, Puustinen MC, Joutsen J, Sistonen L
  TITLE     Uncoupling Stress-Inducible Phosphorylation of Heat Shock Factor 1 
            from Its Activation.
  JOURNAL   Mol Cell Biol. 2015 Jul;35(14):2530-40. doi: 10.1128/MCB.00816-14. 
            Epub 2015 May 11.
  PUBMED    25963659
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00816-14"; journalName: "Mol Cell Biol"; date: 
            "2015-07"; volume: "35"; issue: "14"; pages: "2530-40"
COMMENT     Sequence Label: Gal4-VP16-HSF1 WT
FEATURES             Location/Qualifiers
     source          1..4616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    115..249
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(152..171)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     206..225
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(308..327)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(586..603)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(757..1345)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(837..856)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1517..2308,2309..2377))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2140..2159
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2378..2482)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        2512..2703
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2535..2555)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2553..2850
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2885..3325
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             3833..4066
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(4218..4237)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4262..4310
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 taagtaatga tcataatcag ccatatcaca tctgtagagg ttttacttgc tttaaaaaac
       61 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
      121 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
      181 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
      241 gtctggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
      301 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
      361 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
      421 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
      481 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtgcggtgtg aaataccgca
      541 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
      601 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
      661 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      721 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      781 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      841 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      901 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg
      961 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1021 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1081 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1141 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     1201 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1261 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1321 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1381 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1441 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaaagt atatatgagt
     1501 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     1561 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     1621 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     1681 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     1741 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     1801 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
     1861 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     1921 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     1981 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     2041 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     2101 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
     2161 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     2221 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     2281 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     2341 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     2401 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     2461 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgct tctgaggcgg
     2521 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     2581 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     2641 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     2701 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     2761 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     2821 attccagaag tagtgaagag gcttttttgg aggagatcta agcttgaagc aagcctcctg
     2881 aaagatgaag ctactgtctt ctatcgaaca agcatgcgat atttgccgac ttaaaaagct
     2941 caagtgctcc aaagaaaaac cgaagtgcgc caagtgtctg aagaacaact gggagtgtcg
     3001 ctactctccc aaaaccaaaa ggtctccgct gactagggca catctgacag aagtggaatc
     3061 aaggctagaa agactggaac agctatttct actgattttt cctcgagaag accttgacat
     3121 gattttgaaa atggattctt tacaggatat aaaagcattg ttaacaggat tatttgtaca
     3181 agataatgtg aataaagatg ccgtcacaga tagattggct tcagtggaga ctgatatgcc
     3241 tctaacattg agacagcata gaataagtgc gacatcatca tcggaagaga gtagtaacaa
     3301 aggtcaaaga cagttgactg tatcgccgga attccattcc atgcccaagt atagccggca
     3361 gttctccctg gagcacgtcc acggctcggg cccctactcg gccccctccc cagcctacag
     3421 cagctccagc ctctacgccc ctgatgctgt ggccagctct ggacccatca tctccgacat
     3481 caccgagctg gctcctgcca gccccatggc ctcccccggc gggagcatag acgagaggcc
     3541 cctatccagc agccccctgg tgcgtgtcaa ggaggagccc cccagcccgc ctcagagccc
     3601 ccgggtagag gaggcgagtc ccgggcgccc atcttccgtg gacaccctct tgtccccgac
     3661 cgccctcatt gactccatcc tgcgggagag tgaacctgcc cccgcctccg tcacagccct
     3721 cacggacgcc aggggccaca cggacaccga gggccggcct ccctcccccc cgcccacctc
     3781 cacccctgaa aagtgcctca gcgtagcctg cctggaattc ccggggatct gggccccccc
     3841 gaccgatgtc agcctggggg acgagctcca cttagacggc gaggacgtgg cgatggcgca
     3901 tgccgacgcg ctagacgatt tcgatctgga catgttgggg gacggggatt ccccgggtcc
     3961 gggatttacc ccccacgact ccgcccccta cggcgctctg gatatggccg acttcgagtt
     4021 tgagcagatg tttaccgatg cccttggaat tgacgagtac ggtgggtagg gggcgcgacc
     4081 ggacccgcat cccccgtctg ggttttcccc tcccgtcccc ggttcgtatc cacaataaac
     4141 acgagcacat acattacaaa aacctgcggt tgtcgtctga ttatttggtg gatccggggg
     4201 agatggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     4261 acggcaataa aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg
     4321 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     4381 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
     4441 cgcagccaac gtcggggcgg caggccctgc catagccact ggccccgtgg gttagggacg
     4501 gggtccccca tggggaatgg tttatggttc gtgggggtta ttattttggg cgttgcgtgg
     4561 ggtcaggtcc acgacccaag cttgtcgacg gtaccccggg gaattcgagc tctaga
//
