LOCUS       sequence-141413-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid expressing fusion construct of Gal4 BDB VP16 AD and 
            HSF1-Delta∼PRD RD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Budzynski MA, Puustinen MC, Joutsen J, Sistonen L
  TITLE     Uncoupling Stress-Inducible Phosphorylation of Heat Shock Factor 1 
            from Its Activation.
  JOURNAL   Mol Cell Biol. 2015 Jul;35(14):2530-40. doi: 10.1128/MCB.00816-14. 
            Epub 2015 May 11.
  PUBMED    25963659
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00816-14"; journalName: "Mol Cell Biol"; date: 
            "2015-07"; volume: "35"; issue: "14"; pages: "2530-40"
COMMENT     Sequence Label: Gal4-VP16-HSF1-Delta∼PRD
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(130..363)
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
ORIGIN
        1 ggatccacca aataatcaga cgacaaccgc aggtttttgt aatgtatgtg ctcgtgttta
       61 ttgtggatac gaaccgggga cgggagggga aaacccagac gggggatgcg ggtccggtcg
      121 cgccccctac ccaccgtact cgtcaattcc aagggcatcg gtaaacatct gctcaaactc
      181 gaagtcggcc atatccagag cgccgtaggg ggcggagtcg tggggggtaa atcccggacc
      241 cggggaatcc ccgtccccca acatgtccag atcgaaatcg tctagcgcgt cggcatgcgc
      301 catcgccacg tcctcgccgt ctaagtggag ctcgtccccc aggctgacat cggtcggggg
      361 ggcccagatc cccgggaatt ccaggcaggc tacgctgagg cacttttcag gggcggcggc
      421 gggcgggggt gcgggaggcc ggccctcggt gtccgtgtgg cccctggcgt ccgtgagggc
      481 tgtgacggcg gcgggggcag gttcagcctc ccgcaggatg gagtcaatga gggcggtcgg
      541 ggccaagagg gcgtccacgg cagctgggcg cccgggagcc gcctcctcta cccggggggc
      601 ctgaggcggg gcggggggct cctccttgac acgcaccagg ggggcgctgg ataggggcct
      661 ctcgtctatg ctcccgccgg gggaggccat ggggctggca ggagccagct cggtgatgtc
      721 ggagatgatg ggtccagagc tggccacagc atcaggggcg tagaggctgg agctgctgta
      781 ggctggggag ggggccgagt aggggcccga gccgtggacg tgctccaggg cgaactgccg
      841 gctatacttg ggcatggaat ggaattccgg cgatacagtc aactgtcttt gacctttgtt
      901 actactctct tccgatgatg atgtcgcact tattctatgc tgtctcaatg ttagaggcat
      961 atcagtctcc actg
//
