LOCUS       sequence-141415-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid expressing fusion construct of Gal4 BDB VP16 AD and 
            HSF1-Delta∼PRD RD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Budzynski MA, Puustinen MC, Joutsen J, Sistonen L
  TITLE     Uncoupling Stress-Inducible Phosphorylation of Heat Shock Factor 1 
            from Its Activation.
  JOURNAL   Mol Cell Biol. 2015 Jul;35(14):2530-40. doi: 10.1128/MCB.00816-14. 
            Epub 2015 May 11.
  PUBMED    25963659
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00816-14"; journalName: "Mol Cell Biol"; date: 
            "2015-07"; volume: "35"; issue: "14"; pages: "2530-40"
COMMENT     Sequence Label: Gal4-VP16-HSF1-Delta∼PRD
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             89..529
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 cgaggccgcc tcggcctctg agctattcca gaagtagtga agaggctttt ttggaggaga
       61 tctaagcttg aagcaagcct cctgaaagat gaagctactg tcttctatcg aacaagcatg
      121 cgatatttgc cgacttaaaa agctcaagtg ctccaaagaa aaaccgaagt gcgccaagtg
      181 tctgaagaac aactgggagt gtcgctactc tcccaaaacc aaaaggtctc cgctgactag
      241 ggcacatctg acagaagtgg aatcaaggct agaaagactg gaacagctat ttctactgat
      301 ttttcctcga gaagaccttg acatgatttt gaaaatggat tctttacagg atataaaagc
      361 attgttaaca ggattatttg tacaagataa tgtgaataaa gatgccgtca cagatagatt
      421 ggcttcagtg gagactgata tgcctctaac attgagacag catagaataa gtgcgacatc
      481 atcatcggaa gagagtagta acaaaggtca aagacagttg actgtatcgc cggaattcca
      541 ttccatgccc aagtatagcc ggcagttcgc cctggagcac gtccacggct cgggccccta
      601 ctcggccccc tccccagcct acagcagctc cagcctctac gcccctgatg ctgtggccag
      661 ctctggaccc atcatctccg acatcaccga gctggctcct gccagcccca tggcctcccc
      721 cggcgggagc atagacgaga ggcccctatc cagcgccccc ctggtgcgtg tcaaggagga
      781 gccccccgcc ccgcctcagg ccccccgggt agaggaggcg gctcccgggc gcccagctgc
      841 cgtggacgcc ctcttggccc cgaccgccct cattgactcc atcctgcggg aggctgaacc
      901 tgcccccgcc gccgtcacag ccctcacgga cgccaggggc cacacggaca ccgagggccg
      961 gcctcccgca c
//
