LOCUS       sequence-141418-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  LacZshRNA (mir155 backbone) was inserted into the 3'UTR of EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Wang S, Sengel C, Emerson MM, Cepko CL
  TITLE     A gene regulatory network controls the binary fate decision of rod 
            and bipolar cells in the vertebrate retina.
  JOURNAL   Dev Cell. 2014 Sep 8;30(5):513-27. doi: 
            10.1016/j.devcel.2014.07.018. Epub 2014 Aug 21.
  PUBMED    25155555
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2014.07.018"; journalName: "Dev Cell"; date: 
            "2014-09-8- 8"; volume: "30"; issue: "5"; pages: "513-27"
COMMENT     Sequence Label: CAG-EGFP-LacZshRNA
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     339..358
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    357..412
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     458..477
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     ncRNA           complement(664..690)
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(742..763)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 attacgccaa gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg
       61 actgggagta gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt
      121 agggatgtta aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc
      181 aaggcttttc tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct
      241 ctttatagcc aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa
      301 accaaatact cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag
      361 agacacaaaa aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa
      421 gtcagatgct caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct
      481 cagtggtatt tgtgagccag ggcattggcc acaccagcca ccaccttctg ataggcagcc
      541 tgcacctgag gagtgcggcc gcatttgttc catgtgagtg ctagtaacag gccttgtgtc
      601 ctgaaatcgc tgatgtgtag tcgtcagtca gtggccaaaa cgactacaca aatcagcgat
      661 ttcagcatac agccttcagc aagcctccag gccgctttac ttgtacagct cgtccatgcc
      721 gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt
      781 ggggtctttg ctcagggcgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg
      841 gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc
      901 gatgttgtgg cggatcttga agttcacctt gatgccgttc ttctgcttgt cggccatgat
      961 atagacgttg tggctgttgt agttgt
//
