LOCUS       sequence-141423-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  LacZshRNA (mir155 backbone) was inserted into the 3'UTR of mCherry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Wang S, Sengel C, Emerson MM, Cepko CL
  TITLE     A gene regulatory network controls the binary fate decision of rod 
            and bipolar cells in the vertebrate retina.
  JOURNAL   Dev Cell. 2014 Sep 8;30(5):513-27. doi: 
            10.1016/j.devcel.2014.07.018. Epub 2014 Aug 21.
  PUBMED    25155555
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2014.07.018"; journalName: "Dev Cell"; date: 
            "2014-09-8- 8"; volume: "30"; issue: "5"; pages: "513-27"
COMMENT     Sequence Label: CAG-mCherry-LacZshRNA
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             6..716
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(151..169)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     419..438
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     ncRNA           722..748
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(935..954)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 aattgatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
       61 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
      121 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
      181 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
      241 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
      301 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
      361 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
      421 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
      481 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
      541 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
      601 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
      661 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaagcgg
      721 cctggaggct tgctgaaggc tgtatgctga aatcgctgat ttgtgtagtc gttttggcca
      781 ctgactgacg actacacatc agcgatttca ggacacaagg cctgttacta gcactcacat
      841 ggaacaaatg cggccgcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg
      901 tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg
      961 gggacatcat gaagcccc
//
