LOCUS       sequence-141424-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized LbCpf1 and Lb crRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Toth E, Weinhardt N, Bencsura P, Huszar K, Kulcsar PI, Talas A, 
            Fodor E, Welker E
  TITLE     Cpf1 nucleases demonstrate robust activity to induce DNA 
            modification by exploiting homology directed repair pathways in 
            mammalian cells.
  JOURNAL   Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0.
  PUBMED    27630115
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Direct."; date: "2016-09-14"; volume: "11:46. doi"; pages: " 
            10.1186/s13062-016-0147-0"
COMMENT     Sequence Label: pTE4398
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagctggcta gcgtttaaac ttaagcttgg taccgccacc atgagcaagc tggagaagtt
      121 tacaaactgc tactccctgt ctaagaccct gaggttcaag gccatccctg tgggcaagac
      181 ccaggagaac atcgacaata agcggctgct ggtggaggac gagaagagag ccgaggatta
      241 taagggcgtg aagaagctgc tggatcgcta ctatctgtct tttatcaacg acgtgctgca
      301 cagcatcaag ctgaagaatc tgaacaatta catcagcctg ttccggaaga aaaccagaac
      361 cgagaaggag aataaggagc tggagaacct ggagatcaat ctgcggaagg agatcgccaa
      421 ggccttcaag ggcaacgagg gctacaagtc cctgtttaag aaggatatca tcgagacaat
      481 cctgccagag ttcctggacg ataaggacga gatcgccctg gtgaacagct tcaatggctt
      541 taccacagcc ttcaccggct tctttgataa cagagagaat atgttttccg aggaggccaa
      601 gagcacatcc atcgccttca ggtgtatcaa cgagaatctg acccgctaca tctctaatat
      661 ggacatcttc gagaaggtgg acgccatctt tgataagcac gaggtgcagg agatcaagga
      721 gaagatcctg aacagcgact atgatgtgga ggatttcttt gagggcgagt tctttaactt
      781 tgtgctgaca caggagggca tcgacgtgta ta
//
