LOCUS       sequence-141425-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized LbCpf1 and Lb crRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Toth E, Weinhardt N, Bencsura P, Huszar K, Kulcsar PI, Talas A, 
            Fodor E, Welker E
  TITLE     Cpf1 nucleases demonstrate robust activity to induce DNA 
            modification by exploiting homology directed repair pathways in 
            mammalian cells.
  JOURNAL   Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0.
  PUBMED    27630115
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Direct."; date: "2016-09-14"; volume: "11:46. doi"; pages: " 
            10.1186/s13062-016-0147-0"
COMMENT     Sequence Label: pTE4398
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(46..126)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(133..180)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 agactcgagc ggccgccact gtgctggata tctgcagaat tcttaggcat agtcggggac
       61 atcatatggg tatgcataat caggcacgtc gtagggataa gcgtaatctg gaacatcgta
      121 tgggtaggat ccctttttct tttttgcctg gccggccttt ttcgtggccg ccggcctttt
      181 gtgcttcacg ctggtctggg cgtactccag ccactccttg ttagagatgg cgatcttcac
      241 cttatccagc ttctcgtcct cggccttctt gaactggccg atggcccaca gcacctttct
      301 ggcgatgtta taggcgccat tggcgtcggc gttctttggc aggatggcat tctcctgggc
      361 ctcatagttc cggctatcgt agaagatgcc gtcggagttc ttcacagggc tgatcagaaa
      421 atccacgtcg gtgcggcctg tgatgctgtt ccgcatctgc agcatcaggc tcatcagggc
      481 cataaagcta gagtagaagg ccttgtcgga ctgctcgcac agcagggctc tgatatcgcc
      541 ctgctgataa ttgatgccgt acttgttgaa cagctcctta taggcgctgg tcaggcacac
      601 ctcctcccag tcgaacacgt tgttcttctt aggattccgg aagattctga tccggttgcc
      661 gtaggagtac agcttccact tcttgatgta atcggcgtct gtgcgagaga agttcttata
      721 gtccagggca aactcgaaca gatcctcctc gggcacgtac atgatcctgt caaaggagct
      781 gatgaacttc ttggaa
//
