LOCUS       sequence-141430-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for Gateway based on vector #395 from the 
            Tol2Kit, containing a gRNA scaffold under the control of a zebrafish
            U6 promoter, and cmlc2:GFP as a transgenesis marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Ablain J, Durand EM, Yang S, Zhou Y, Zon LI
  TITLE     A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in 
            Zebrafish.
  JOURNAL   Dev Cell. 2015 Mar 4. pii: S1534-5807(15)00075-1. doi: 
            10.1016/j.devcel.2015.01.032.
  PUBMED    25752963
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2015 Mar 4. pii: S1534-5807(15)00075-1. doi: 
            10.1016/j.devcel.2015.01.032."
COMMENT     Sequence Label: pDestTol2CG2-U6:gRNA
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..36
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     276..297
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg
       61 gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
      121 gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg
      181 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc
      241 gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat
      301 gggcaccacc ccggtgaaca gctcttcgcc cttgctcacc atggtcactg tctgctttgc
      361 tgttggtctg ggctcctggg tcactggctt actaatggag tctttatgta tgaggactct
      421 tatcaattgt tcttctataa aggtctgcag tgtttctgtt cgtcccctac atggacaccc
      481 agagcctcct aaatacagga gccctgataa ctgcacaagt gctcagattc cagcagggtg
      541 gaaatgagat aaagtgtgca gatggggagg gggacgtgaa tgagagattt gagggatgaa
      601 aaggatggat gaacgcattg aaaatagccc ctttcacaaa ataataccag taaattgtca
      661 tataattaat gaccagacct ttacctgtaa atgatgtgat gttcatatac agaagaatgc
      721 caacaattta cagatgattt tacaaattct taattt
//
