LOCUS       sequence-141432-         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for Gateway based on vector #395 from the 
            Tol2Kit, containing a gRNA scaffold under the control of a zebrafish
            U6 promoter, and cmlc2:GFP as a transgenesis marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Ablain J, Durand EM, Yang S, Zhou Y, Zon LI
  TITLE     A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in 
            Zebrafish.
  JOURNAL   Dev Cell. 2015 Mar 4. pii: S1534-5807(15)00075-1. doi: 
            10.1016/j.devcel.2015.01.032.
  PUBMED    25752963
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2015 Mar 4. pii: S1534-5807(15)00075-1. doi: 
            10.1016/j.devcel.2015.01.032."
COMMENT     Sequence Label: pDestTol2CG2-U6:gRNA
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    71..92
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(107..112,113..130,131..137)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    145..161
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     150..172
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     169..185
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     169..185
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     204..224
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        206..224
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     261..277
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     261..277
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(311..322,323..510)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
       61 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
      121 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
      181 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
      241 tccaccgcgg tggcggccgc tctagaacta gtggatctgc tgggcttgct gaaggtaggg
      301 ggtcaagaac cagaggtgta aagtacttga gtaattttac ttgattactg tacttaagta
      361 ttatttttgg ggatttttac tttacttgag tacaattaaa aatcaatact tttactttta
      421 cttaattaca tttttttaga aaaaaaagta ctttttactc cttacaattt tatttacagt
      481 caaaaagtac ttattttttg gagatcactt cattctattt tcccttgcta ttaccaaacc
      541 aattgaattg cgctgatgcc cagtttaatt taaatgttat ttattctgcc tatgaaaatc
      601 gttttcacat tatatgaaat tggtcagaca tgttcattgg tcctttggaa gtgacgtcat
      661 gtcacatcta ttaccacaat gcacagcacc ttgacctgga
//
