LOCUS       sequence-141433-         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for Gateway based on vector #395 from the 
            Tol2Kit, containing a gRNA scaffold under the control of a zebrafish
            U6 promoter, and cmlc2:GFP as a transgenesis marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Ablain J, Durand EM, Yang S, Zhou Y, Zon LI
  TITLE     A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in 
            Zebrafish.
  JOURNAL   Dev Cell. 2015 Mar 4. pii: S1534-5807(15)00075-1. doi: 
            10.1016/j.devcel.2015.01.032.
  PUBMED    25752963
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2015 Mar 4. pii: S1534-5807(15)00075-1. doi: 
            10.1016/j.devcel.2015.01.032."
COMMENT     Sequence Label: pDestTol2CG2-U6:gRNA
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccttgctcac catggtcact gtctgctttg ctgttggtct gggctcctgg gtcactggct
       61 tactaatgga gtctttatgt atgaggactc ttatcaattg ttcttctata aaggtctgca
      121 gtgtttctgt tcgtccccta catggacacc cagagcctcc taaatacagg agccctgata
      181 actgcacaag tgctcagatt ccagcagggt ggaaatgaga taaagtgtgc agatggggag
      241 ggggacgtga atgagagatt tgagggatga aaaggatgga tgaacgcatt gaaaatagcc
      301 cctttcacaa aataatacca gtaaattgtc atataattaa tgaccagacc tttacctgta
      361 aatgatgtga tgttcatata cagaagaatg ccaacaattt acagatgatt ttacaaattc
      421 ttaattttcc ttttctaaat ggatttgttc acacattatc tcttcaaagc aattgaattt
      481 tctggaaaag attggctgtg tgtgtaaaag gtgctactga ggaatttaat gtgacatgga
      541 atgaagcaaa cagcagccta agacaggatg gcaggaaaaa tgtcacatgt ttaaaataag
      601 agtgaaacca acgacctgga cttatagaga ctgtgtgtcc ttaggcaaag ctcttgtaca
      661 aacagtgctt gtgactgtga aattcggggt ttgcctggat tgtgttaaat gtgtgcttgg
      721 atgtatcata aa
//
