LOCUS       sequence-141434-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for Gateway based on vector #395 from the 
            Tol2Kit, containing a gRNA scaffold under the control of a zebrafish
            U6 promoter, and cmlc2:GFP as a transgenesis marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Ablain J, Durand EM, Yang S, Zhou Y, Zon LI
  TITLE     A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in 
            Zebrafish.
  JOURNAL   Dev Cell. 2015 Mar 4. pii: S1534-5807(15)00075-1. doi: 
            10.1016/j.devcel.2015.01.032.
  PUBMED    25752963
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2015 Mar 4. pii: S1534-5807(15)00075-1. doi: 
            10.1016/j.devcel.2015.01.032."
COMMENT     Sequence Label: pDestTol2CG2-U6:gRNA
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(68..79,80..242)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     615..631
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     615..631
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    651..774
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atatctgttc agacacccat atccactctg ttccacacag gtcagaggtt tgtccaggag
       61 ttcttgacag aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt
      121 agggaaaatt ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa
      181 gtaaaaaagt actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag
      241 aaagaaaact agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca
      301 tacaaggttt tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac
      361 aatctaatgc cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa
      421 gctgattctc aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg
      481 cagtgctgaa aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag
      541 agaggctgca aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca
      601 gatgggccct cgagcaggaa acagctatga ccatgattac gccaagctat caactttgta
      661 tagaaaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
      721 gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac tatggtcgac
      781 ctgcagactg gctgtgtata agggagcctg acattt
//
