LOCUS       sequence-141438-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Otx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of 
            mCherry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Wang S, Sengel C, Emerson MM, Cepko CL
  TITLE     A gene regulatory network controls the binary fate decision of rod 
            and bipolar cells in the vertebrate retina.
  JOURNAL   Dev Cell. 2014 Sep 8;30(5):513-27. doi: 
            10.1016/j.devcel.2014.07.018. Epub 2014 Aug 21.
  PUBMED    25155555
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2014.07.018"; journalName: "Dev Cell"; date: 
            "2014-09-8- 8"; volume: "30"; issue: "5"; pages: "513-27"
COMMENT     Sequence Label: CAG-mCherry-Otx2shRNA1
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     343..362
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    361..416
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     462..481
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     ncRNA           complement(668..694)
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(978..997)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 catgattacg ccaagcttgg gctgcaggtc gagggatctc cataagagaa gagggacagc
       61 tatgactggg agtagtcagg agaggaggaa aaatctggct agtaaaacat gtaaggaaaa
      121 ttttagggat gttaaagaaa aaaataacac aaaacaaaat ataaaaaaaa tctaacctca
      181 agtcaaggct tttctatgga ataaggaatg gacagcaggg ggctgtttca tatactgatg
      241 acctctttat agccaacctt tgttcatggc agccagcata tgggcatatg ttgccaaact
      301 ctaaaccaaa tactcattct gatgttttaa atgatttgcc ctcccatatg tccttccgag
      361 tgagagacac aaaaaattcc aacacactat tgcaatgaaa ataaatttcc tttattagcc
      421 agaagtcaga tgctcaaggg gcttcatgat gtccccataa tttttggcag agggaaaaag
      481 atctcagtgg tatttgtgag ccagggcatt ggccacacca gccaccacct tctgataggc
      541 agcctgcacc tgaggagtgc ggccgcattt gttccatgtg agtgctagta acaggccttg
      601 tgtcctgaat ccaagcagag cattgagtca gtcagtggcc aaaactcaat gctgactgct
      661 tggattcagc atacagcctt cagcaagcct ccaggccgct tacttgtaca gctcgtccat
      721 gccgccggtg gagtggcggc cctcggcgcg ttcgtactgt tccacgatgg tgtagtcctc
      781 gttgtgggag gtgatgtcca acttgatgtt gacgttgtag gcgccgggca gctgcacggg
      841 cttcttggcc ttgtaggtgg tcttgacctc agcgtcgtag tggccgccgt ccttcagctt
      901 cagcctctgc ttgatctcgc ccttcagggc gccgtcctcg gggtacatcc gctcggagga
      961 ggcctcccag cccatggtct tcttctgcat tacggggccg tcggagggga
//
