LOCUS       sequence-141440-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for Gateway based on vector #394 from the 
            Tol2Kit, containing a gRNA scaffold under the control of a zebrafish
            U6 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Ablain J, Durand EM, Yang S, Zhou Y, Zon LI
  TITLE     A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in 
            Zebrafish.
  JOURNAL   Dev Cell. 2015 Mar 4. pii: S1534-5807(15)00075-1. doi: 
            10.1016/j.devcel.2015.01.032.
  PUBMED    25752963
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2015 Mar 4. pii: S1534-5807(15)00075-1. doi: 
            10.1016/j.devcel.2015.01.032."
COMMENT     Sequence Label: pDestTol2pA2-U6:gRNA
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(109..232)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(252..268)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(252..268)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    join(641..803,804..815)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
       61 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
      121 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
      181 ttgatattta tatcatttta cgtttctcgt tcaacttttc tatacaaagt tgatagcttg
      241 gcgtaatcat ggtcatagct gtttcctgct cgagggccca tctggcctgt gtttcagaca
      301 ccagggagtc tctgctcacg tttcctgcta tttgcagcct ctctatcaag actaatacac
      361 ctcttcccgc atcggctgcc tgtgagaggc ttttcagcac tgcaggattg cttttcagcc
      421 ccaaaagagc taggcttgac actaacaatt ttgagaatca gcttctactg aagttaaatc
      481 tgaggtttta caactttgag tagcgtgtac tggcattaga ttgtctgtct tatagtttga
      541 taattaaata caaacagttc taaagcagga taaaaccttg tatgcatttc atttaatgtt
      601 ttttgagatt aaaagcttaa acaagaatct ctagttttct ttcttgcttt tacttttact
      661 tccttaatac tcaagtacaa ttttaatgga gtactttttt acttttactc aagtaagatt
      721 ctagccagat acttttactt ttaattgagt aaaattttcc ctaagtactt gtactttcac
      781 ttgagtaaaa tttttgagta ctttttacac ctctgtc
//
