LOCUS       sequence-141452-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Crx shRNA (mir30 backbone) was inserted into the 3'UTR of mCherry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Wang S, Sengel C, Emerson MM, Cepko CL
  TITLE     A gene regulatory network controls the binary fate decision of rod 
            and bipolar cells in the vertebrate retina.
  JOURNAL   Dev Cell. 2014 Sep 8;30(5):513-27. doi: 
            10.1016/j.devcel.2014.07.018. Epub 2014 Aug 21.
  PUBMED    25155555
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2014.07.018"; journalName: "Dev Cell"; date: 
            "2014-09-8- 8"; volume: "30"; issue: "5"; pages: "513-27"
COMMENT     Sequence Label: CAG-mCherry-CrxshRNA
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     342..361
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    360..415
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     461..480
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     ncRNA           complement(566..694)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(722..736)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 atgattacgc caagcttggg ctgcaggtcg agggatctcc ataagagaag agggacagct
       61 atgactggga gtagtcagga gaggaggaaa aatctggcta gtaaaacatg taaggaaaat
      121 tttagggatg ttaaagaaaa aaataacaca aaacaaaata taaaaaaaat ctaacctcaa
      181 gtcaaggctt ttctatggaa taaggaatgg acagcagggg gctgtttcat atactgatga
      241 cctctttata gccaaccttt gttcatggca gccagcatat gggcatatgt tgccaaactc
      301 taaaccaaat actcattctg atgttttaaa tgatttgccc tcccatatgt ccttccgagt
      361 gagagacaca aaaaattcca acacactatt gcaatgaaaa taaatttcct ttattagcca
      421 gaagtcagat gctcaagggg cttcatgatg tccccataat ttttggcaga gggaaaaaga
      481 tctcagtggt atttgtgagc cagggcattg gccacaccag ccaccacctt ctgataggca
      541 gcctgcacct gaggagtgcg gccgcaaagt gatttaattt ataccatttt aattcagctt
      601 tgtaaaaatg tatcaaagag atagcaaggt attcagtttt agtaaacaag ataattgctc
      661 ctaaagtagc cccttgaatt ctccgaggca gtaggcacac caggctgtcc catactcaat
      721 acatctgtgg cttcactatt gagtatggga cagcctggtt cgctcactgt caacag
//
