LOCUS       sequence-141455-         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Otx2 shRNA2 (mir155 backbone) was inserted into the 3'UTR of 
            mCherry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Wang S, Sengel C, Emerson MM, Cepko CL
  TITLE     A gene regulatory network controls the binary fate decision of rod 
            and bipolar cells in the vertebrate retina.
  JOURNAL   Dev Cell. 2014 Sep 8;30(5):513-27. doi: 
            10.1016/j.devcel.2014.07.018. Epub 2014 Aug 21.
  PUBMED    25155555
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2014.07.018"; journalName: "Dev Cell"; date: 
            "2014-09-8- 8"; volume: "30"; issue: "5"; pages: "513-27"
COMMENT     Sequence Label: CAG-mCherry-Otx2shRNA2
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     342..361
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    360..415
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     461..480
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     ncRNA           complement(667..693)
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 atgattacgc caagcttggg ctgcaggtcg agggatctcc ataagagaag agggacagct
       61 atgactggga gtagtcagga gaggaggaaa aatctggcta gtaaaacatg taaggaaaat
      121 tttagggatg ttaaagaaaa aaataacaca aaacaaaata taaaaaaaat ctaacctcaa
      181 gtcaaggctt ttctatggaa taaggaatgg acagcagggg gctgtttcat atactgatga
      241 cctctttata gccaaccttt gttcatggca gccagcatat gggcatatgt tgccaaactc
      301 taaaccaaat actcattctg atgttttaaa tgatttgccc tcccatatgt ccttccgagt
      361 gagagacaca aaaaattcca acacactatt gcaatgaaaa taaatttcct ttattagcca
      421 gaagtcagat gctcaagggg cttcatgatg tccccataat ttttggcaga gggaaaaaga
      481 tctcagtggt atttgtgagc cagggcattg gccacaccag ccaccacctt ctgataggca
      541 gcctgcacct gaggagtgcg gccgcatttg ttccatgtga gtgctagtaa caggccttgt
      601 gtcctgataa cctgaagtga gtatagtcag tcagtggcca aaactatact caggcttcag
      661 gttatcagca tacagccttc agcaagcctc caggccgctt acttgtacag ctcgtccatg
      721 ccgccggtgg agtggcggcc ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg
      781 ttgtgggagg tgatgtccaa cttgatgttg acgttgtagg cgccgggcag ctgcacgggc
      841 ttcttggcct tgtaggtggt cttgacctca gcgtcgtagt ggccgccgtc cttcagcttc
      901 agcctctgct tgatctcgcc cttcagggcg ccgtcctcgg gg
//
