LOCUS       sequence-141477-       11678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for expression of gene by 
            2xCaMV35S enhancer and omega sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11678)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 11678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB402-omega
FEATURES             Location/Qualifiers
     source          1..11678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        405..1083
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     1020..1040
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1172..1296
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1333..1338,1339..1356,1357..1363)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1417..2076
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1483..1502)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2418..2723
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2602..2621
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2764..2888)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2920..3172
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3206..3222)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3206..3222)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3219..3241)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3230..3246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3254..3260,3261..3278,3279..3284))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3299..3320
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3354..3606)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3996..4790)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4107..4126)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4717..4736
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4811..4994)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(4847..5026)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5200..5224
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5745..6536
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6782..7370
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7271..7290
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7524..7541
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7556..7696
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7622..7641)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7782..7804
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8040..8234
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8300..9373)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9802..10431)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac
      361 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
      421 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
      481 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
      541 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
      601 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac
      661 atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac
      721 caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat
      781 tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa
      841 tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc
      901 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
      961 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac
     1021 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacgc
     1081 tgaaatcacc agtgtatttt tacaacaatt accaacaaca acaaacaaca aacaacatta
     1141 caattactat ttacaatttc tagagttatc aacaagtttg tacaaaaaag ctgaacgaga
     1201 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     1261 ataatactgt aaaacacaac atatccagtc actatggcgg cccctcgagg ggccgcatta
     1321 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
     1381 gatccggcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     1441 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     1501 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     1561 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     1621 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     1681 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     1741 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     1801 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     1861 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     1921 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     1981 attcaggttc atcatgccgt ctgtgatggc ttccatgtcg gcagaatgct taatgaatta
     2041 caacagtact gcgatgagtg gcagggcggg gcgtaaagat ctggatccgg cttactaaaa
     2101 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     2161 atgtataccc gaagtatgtc aaaaagaggt gtgctatgaa gcagcgtatt acagtgacag
     2221 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
     2281 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
     2341 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
     2401 gaacagggac tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
     2461 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
     2521 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     2581 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     2641 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     2701 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
     2761 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     2821 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
     2881 aaagtggttg ataacagcgc ttagagctcg aatttccccg atcgttcaaa catttggcaa
     2941 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     3001 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     3061 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     3121 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg
     3181 gttccggaac caattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3241 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3301 gagtgagcta actcacatta ggctgaatta ggcgcgccta tttctgaatt cccgatctag
     3361 taacatagat gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc
     3421 tatcgcgtat taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa
     3481 cgtcatgcat tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat
     3541 aatcatcgca agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga
     3601 acgatcgggg atcatccggg tctgtggcgg gaactccacg aaaatatccg aacgcagcaa
     3661 gatatcgcgg tgcatctcgg tcttgcctgg gcagtcgccg ccgacgccgt tgatgtggac
     3721 gccgggcccg atcatattgt cgctcaggat cgtggcgttg tgcttgtcgg ccgttgctgt
     3781 cgtaatgata tcggcacctt cgaccgcctg ttccgcagag atcccgtggg cgaagaactc
     3841 cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa cgattccgaa
     3901 gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtgatggca ggttgggcgt
     3961 cgcttggtcg gtcatttcga accccagagt cccgctcaga agaactcgtc aagaaggcga
     4021 tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca
     4081 gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag
     4141 cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc
     4201 atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcatcgcc gtcgggcatg
     4261 cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga
     4321 tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc
     4381 gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca
     4441 gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc
     4501 acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg
     4561 caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg cagttcattc
     4621 agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg
     4681 aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc
     4741 tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat
     4801 ccagatccgg tgcagattat ttggattgag agtgaatatg agactctaat tggataccga
     4861 ggggaattta tggaacgtca gtggagcatt tttgacaaga aatatttgct agctgatagt
     4921 gaccttaggc gacttttgaa cgcgcaataa tggtttctga cgtatgtgct tagctcatta
     4981 aactccagaa acccgcggct gagtggctcc ttcaacgttg cggttctgtc agttccaaac
     5041 gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac gtgactccct taattctccg
     5101 ctcatgatga attcagaaat aaattcagcc taattcggcg ttaattcagt acattaaaaa
     5161 cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct
     5221 gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac
     5281 aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt
     5341 gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg
     5401 atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat
     5461 caattcgggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     5521 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     5581 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     5641 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     5701 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgggg gaagcggtga
     5761 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     5821 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     5881 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     5941 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     6001 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     6061 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     6121 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     6181 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     6241 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     6301 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     6361 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     6421 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     6481 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc
     6541 tagctagaaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga
     6601 tgcactaagc acataattgc tcacagccaa actatcaggt caagtctgct tttattattt
     6661 ttaagcgtgc ataataagcc ctacacaaat tgggagatat atcatgcatg accaaaatcc
     6721 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6781 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6841 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6901 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     6961 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     7021 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     7081 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     7141 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     7201 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     7261 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     7321 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     7381 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     7441 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     7501 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     7561 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     7621 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
     7681 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     7741 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     7801 gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc
     7861 ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag
     7921 ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc
     7981 gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt
     8041 atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa
     8101 aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg
     8161 gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt
     8221 acgtgctatc cacaggaaag agaccttttc gacctttttc ccctgctagg gcaatttgcc
     8281 ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt
     8341 agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca
     8401 ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc
     8461 tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc
     8521 ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt
     8581 gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta
     8641 gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa
     8701 tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct
     8761 gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct
     8821 ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc
     8881 ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa
     8941 ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa
     9001 cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct
     9061 tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat
     9121 agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg
     9181 gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc
     9241 acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct
     9301 tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt
     9361 tgcgaccgct cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca
     9421 gacaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac
     9481 ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca
     9541 tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca
     9601 gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg
     9661 ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg
     9721 cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca
     9781 ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg
     9841 gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc
     9901 agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc
     9961 ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac
    10021 aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag
    10081 gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc
    10141 tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa
    10201 gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac
    10261 ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga
    10321 atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat
    10381 ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc
    10441 ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg
    10501 ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag
    10561 ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc
    10621 acggttgaga cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg
    10681 atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt
    10741 cctcttggcg ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac
    10801 ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc
    10861 ggtacagggt cgagcgatgc acgccaagca gtgcagccgc ctctttcacg gtgcggcctt
    10921 cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc
    10981 caaacttcac gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta
    11041 gggaacgctc gaactcggca atgccggcga acacggtcaa caccatgcgg ccggccggcg
    11101 tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc
    11161 gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg
    11221 tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc
    11281 tggtgccggt gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc
    11341 gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg
    11401 cggcgctctt gttcatggcg taatgtctcc ggttctagtc gcaagtattc tactttatgc
    11461 gactaaaaca cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa
    11521 cttaggactt gtgcgacatg tcgttttcag aagacggctg cactgaacgt cagaagccga
    11581 ctgcactata gcagcggagg ggttggatca aagtactttg atcccgaggg gaaccctgtg
    11641 gttggcatgc acatacaaat ggacgaacgg ataaacct
//
