LOCUS       sequence-141491-       11856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            4xMyc (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11856)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 11856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB417
FEATURES             Location/Qualifiers
     source          1..11856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1179..1303
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1340..1345,1346..1363,1364..1370)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1424..2083
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1490..1509)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2425..2730
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2609..2628
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2771..2895)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2921..2950
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2957..2986
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3002..3031
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3038..3067
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      3098..3350
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3384..3400)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3384..3400)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3397..3419)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3408..3424
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3432..3438,3439..3456,3457..3462))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3477..3498
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3532..3784)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4174..4968)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4285..4304)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4895..4914
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4989..5172)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(5025..5204)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5378..5402
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5923..6714
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6960..7548
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7449..7468
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7702..7719
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7734..7874
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7800..7819)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7960..7982
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8218..8412
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8478..9551)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9980..10609)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccggcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa cagggactgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcgggtt aattaacggt gaacaaaagc taatctccga
     2941 ggaagacttg aacggtgaac aaaaattaat ctcagaagaa gacttgaacg gactcgacgg
     3001 tgaacaaaag ttgatttctg aagaagattt gaacggtgaa caaaagctaa tctccgagga
     3061 agacttgaac ggtagcgctt agagctcgaa tttccccgat cgttcaaaca tttggcaata
     3121 aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
     3181 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     3241 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     3301 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattggt
     3361 tccggaacca attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     3421 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     3481 gtgagctaac tcacattagg ctgaattagg cgcgcctatt tctgaattcc cgatctagta
     3541 acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta
     3601 tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg
     3661 tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa
     3721 tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac
     3781 gatcggggat catccgggtc tgtggcggga actccacgaa aatatccgaa cgcagcaaga
     3841 tatcgcggtg catctcggtc ttgcctgggc agtcgccgcc gacgccgttg atgtggacgc
     3901 cgggcccgat catattgtcg ctcaggatcg tggcgttgtg cttgtcggcc gttgctgtcg
     3961 taatgatatc ggcaccttcg accgcctgtt ccgcagagat cccgtgggcg aagaactcca
     4021 gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc
     4081 ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg
     4141 cttggtcggt catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata
     4201 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
     4261 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
     4321 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
     4381 gatattcggc aagcaggcat cgccatgggt cacgacgaga tcatcgccgt cgggcatgcg
     4441 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc
     4501 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc
     4561 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc
     4621 catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac
     4681 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca
     4741 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag
     4801 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa
     4861 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc
     4921 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc
     4981 agatccggtg cagattattt ggattgagag tgaatatgag actctaattg gataccgagg
     5041 ggaatttatg gaacgtcagt ggagcatttt tgacaagaaa tatttgctag ctgatagtga
     5101 ccttaggcga cttttgaacg cgcaataatg gtttctgacg tatgtgctta gctcattaaa
     5161 ctccagaaac ccgcggctga gtggctcctt caacgttgcg gttctgtcag ttccaaacgt
     5221 aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt gactccctta attctccgct
     5281 catgatgaat tcagaaataa attcagccta attcggcgtt aattcagtac attaaaaacg
     5341 tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc
     5401 caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag
     5461 gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc
     5521 tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat
     5581 gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca
     5641 attcgggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
     5701 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     5761 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
     5821 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     5881 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc
     5941 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     6001 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
     6061 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     6121 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     6181 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     6241 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     6301 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     6361 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     6421 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     6481 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     6541 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     6601 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     6661 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta
     6721 gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg
     6781 cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt
     6841 aagcgtgcat aataagccct acacaaattg ggagatatat catgcatgac caaaatccct
     6901 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6961 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7021 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7081 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     7141 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7201 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7261 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7321 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7381 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7441 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7501 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7561 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     7621 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     7681 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     7741 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     7801 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     7861 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     7921 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     7981 ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg
     8041 tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc
     8101 atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc
     8161 agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat
     8221 gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa
     8281 tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc
     8341 tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac
     8401 gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct
     8461 agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag
     8521 cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg
     8581 tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg
     8641 ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg
     8701 cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga
     8761 accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc
     8821 tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc
     8881 aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc
     8941 atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt
     9001 ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg
     9061 ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc
     9121 gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc
     9181 tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc
     9241 gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag
     9301 agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc
     9361 gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac
     9421 gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc
     9481 aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg
     9541 cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga
     9601 caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg
     9661 cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc
     9721 tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc
     9781 tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc
     9841 ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca
     9901 gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt
     9961 ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga
    10021 cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag
    10081 tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg
    10141 ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa
    10201 aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt
    10261 cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg
    10321 ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt
    10381 cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg
    10441 ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat
    10501 cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg
    10561 tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt
    10621 gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt
    10681 ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct
    10741 ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac
    10801 ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat
    10861 ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc
    10921 tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg
    10981 aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg
    11041 tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc
    11101 tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca
    11161 aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg
    11221 gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg
    11281 gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc
    11341 tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc
    11401 acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg
    11461 gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt
    11521 tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg
    11581 gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga
    11641 ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact
    11701 taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact
    11761 gcactatagc agcggagggg ttggatcaaa gtactttgat cccgagggga accctgtggt
    11821 tggcatgcac atacaaatgg acgaacggat aaacct
//
